Multiphoton Laser Scanning Microscope (MZ1 - Zeiss 2photon upright) Zeiss Axio Examiner.Z1

Basic Information

Name:	Multiphoton Laser Scanning Microscope (MZ1 - Zeiss 2photon upright)
Manufacturer:	Zeiss
Model:	Axio Examiner.Z1
Facility:	Light Microscopy (LMF)
Partner:	Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG)
Inventory number:	AIM System 2501000445 10.1.145.41 (IPv4)

Overview: Zeiss LSM 780 NLOMultiphoton Laser Scanning Microscope with a tunable pulsed infra-red laser (Chameleon Vision II) for multiphoton excitation.Single photon point scanning confocal system with three PMTs (one spectral GaAsP detector) and transmitted light detector. Motorized stage for multi-point acquisition"Zeiss LSM 780 NLO
Multiphoton Laser Scanning Microscope with a tunable pulsed infra-red laser (Chameleon Vision II) for multiphoton excitation.
Single photon point scanning confocal system with three PMTs (one spectral GaAsP detector) and transmitted light detector. Motorized stage for multi-point acquisition" cannot be used as a page name in this wiki.; Suitable for imaging of thick specimens fast bleaching samples. Spectral imaging, routine dye combinations, tile scan, multi position scan, FCS

Features: Upright stand; motorized XY stage; motorized Z-drive,; simultaneous imaging; sequential imaging; line scan; point scan; Z stacks; time-series; advanced time-series; averaging; summarizing; scan zoom and rotation; tile scan; multi position scan; spectral scan

Objectives: Zeiss EC Plan-Neofluar 10x 0.3; Zeiss Plan-Apochromat 10x 0.45; Zeiss Clr Plan-Apochromat 10x 0.5 Scale; Zeiss N-Achroplan 20x 0.5 W; Zeiss Plan-Apochromat 20x 0.8; Zeiss W Plan-Apochromat 20x 1.0 DIC; Zeiss Clr Plan-Neofluar 20x 1.0 Korr Clarity; Zeiss LD SC Plan-Apochromat 20x 1.0 Korr Scale; Zeiss LD LCI Plan-Apochromat 25x 0.8 Oil/Glyc/Water DIC; Zeiss C-Apochromat 40x 1.2 W

Illumination: Fluorescence (Metal Halide, HXP, 120W); Transmitted Light (Halogen); Laser Diode 405 nm; Laser Argon Multiline 458, 488, 514 nm; Laser DPSS 561 nm; Laser HeNe 594 nm; Laser HeNe 633 nm; Laser Multiphoton Chameleon 700-1064 nm

Detection: Single photon detection: 2 confocal PMTs with enhanced detection in the red range; GaAsP spectral detector with 32 channels; T-PMT; dichromatic mirrors for excitation/emission splitting; spectral prism sliders for emission detection range selection; common pinhole changeable for each detector setting; 2 photon detection: 4 non-descanned GaAsP detectors (2 BiG units) in reflected direction; 2 non-descanned PMTs (1 NDD moule) in transmitted direction