Multiphoton Laser Scanning Microscope (MZ2 - Zeiss 2photon inverted) Zeiss Axio Observer

Basic Information

Name:	Multiphoton Laser Scanning Microscope (MZ2 - Zeiss 2photon inverted)
Manufacturer:	Zeiss
Model:	Axio Observer
Facility:	Light Microscopy (LMF)
Partner:	Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG)
Inventory number:	AIM-System 2501000455 10.1.30.42

Overview: Zeiss LSM 710 NLOMultiphoton Laser Scanning Microscope with a tunable pulsed infra-red laser (Chameleon Vision II) for multi-photon excitation.Single photon point scanning confocal system with three PMTs (one spectral) and transmitted light detector. Motorized stage for multi-point acquisition"Zeiss LSM 710 NLO
Multiphoton Laser Scanning Microscope with a tunable pulsed infra-red laser (Chameleon Vision II) for multi-photon excitation.
Single photon point scanning confocal system with three PMTs (one spectral) and transmitted light detector. Motorized stage for multi-point acquisition" cannot be used as a page name in this wiki.; Suitable for imaging of thick specimens fast bleaching samples. Spectral imaging, routine dye combinations, tile scan, multi position scan.

Features: Inverted stand; motorized XY stage; motorized Z-drive,; simultaneous imaging; sequential imaging; line scan; point scan; Z stacks; time-series; advanced time-series; averaging; summarizing; scan zoom and rotation; tile scan; multi position scan; spectral scan

Objectives: Zeiss Plan-Apochromat 10x 0.45; Zeiss C-Apochromat 40x 1.2 W; Zeiss Plan-Apochromat 20x 0.8; Zeiss Plan-Apochromat 63x 1.4 Oil; Zeiss LD C-Apochromat 40x 1.1 W; Zeiss LD LCI Plan-Apochromat 25x 0.8 Oil/Glyc/Water DIC; Zeiss LCI Plan-Neofluar 63x 1.3 Gly/Water DIC

Illumination: Fluorescence (Metal Halide, PRIOR, 200W); Transmitted Light (Halogen); Laser Diode 405 nm; Laser Argon Multiline 458, 477, 488, 514 nm; Laser DPSS 561 nm; Laser HeNe 594 nm; Laser HeNe 633 nm; Laser Multiphoton Chameleon 700-1064 nm

Detection: [[detection::Single photon detection: 2 confocal PMTs; QUASAR detector with 32 channels; T-PMT; dichromatic mirrors for excitation/emission splitting; spectral prism sliders for emission detection range selection; common pinhole changeable for each detector setting; 2 photon detection: 2 non-descanned detectors (NDD) - NDD-dichromatic mirrors and emission filters]]