View all instruments of this unit Real-Time Confocal Microscope (SD3 - Zeiss Spinning Disc) Zeiss Axio Observer
Basic Information
| Name: | Real-Time Confocal Microscope (SD3 - Zeiss Spinning Disc) | |
| Manufacturer: | Zeiss | |
| Model: | Axio Observer | |
| Facility: | Light Microscopy (LMF) |
|
| Partner: | Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG) |
|
| Inventory number: | MOZG04081820000 10.1.145.37 | |
Description
- Category
- Light Microscope
- Overview
- Fast, "real-time" confocal imaging with DirectFRAP
- Suitable for fast live confocal imaging
- Features
- Inverted stand
- motorized with Definite Focus (hardware autofocus)
- 1.6x Optovar
- Prior NanoScanZ
- Märzhäuser xy scanning stage
- 63x 1.3 Imm Korr lens - AxioCamMR3 - 2x binning
- Pix spacing = 204.762 nm
- Pinhole spacing is 250um and pinhole size is 50um diameter
- Objectives
- Zeiss Plan-Apochromat 10x 0.45
- Zeiss C-Apochromat 63x 1.2 W
- Zeiss LCI Plan-Neofluar 63x 1.3 Gly/Water DIC
- Zeiss Alpha Plan-Apochromat 100x 1.46 Oil DIC
- Zeiss Plan-Apochromat 40x 1.3 Oil DIC
- Zeiss LD LCI Plan-Apochromat 25x 0.8 Oil/Glyc/Water DIC
- Zeiss LD C-Apochromat 40x 1.1 W
- Zeiss Plan-Apochromat 63x 1.4 Oil DIC
- Illumination
- Transmitted Light (LED)
- Fluorescence (Metal Halide, HXP, 120W)
- Laser Diode 405 nm
- Laser DPSS 445 nm
- Laser DPSS 488 nm
- Laser DPSS 520nm
- Laser DPSS 561 nm
- Laser DPSS 639nm
- Detection
- Spinning disc scan head Yokogawa CSU-X1 with two camera ports
- pinhole radius: 25um
- pinhole spacing: 250um;
- 3 DCs: (1) RTFT 405+488+561
- (2) RQFT 405+488+568+647
- (3) RTFT 457+514+647
- Zeiss AxiCam MRm with EM filter wheel: (1) BP 450/50
- (2) 458LP
- (3) DBP 527/54 + 645/60
- (4) 535/30
- (5) 629/62
- (6) BP 690/50;
- Roper evolve 515 EMCCD with manual EM filter holder
- Reflectors
- Pos 1: empty for use with CSU
- Pos 2: FS49: Ex 365
- BS 395
- EM BP445/50
Pos 3: FS38HE: Ex BP470/40 - BS 495
- EM BP525/50
Pos 4: FS43HE: Ex BP550/25 - BS 570
- EM BP605/70
Pos 5: FS50: Ex BP640/30 - BS 660
- EM BP690/50
Pos 6: 80/20 mirror for FRAP
- Software
- Zeiss ZEN 2 Blue
- Incubation
- Cage incubator (T+CO2)
Link to Further Details
Points of Contact
Images
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MPI-SD3.jpg |
Last Update
Last updated at: 8 April 2018 at 03:11:07