Single Plane Illumination Microscope (LZ1 - Zeiss Lightsheet Z.1) Zeiss LightSheet LZ1 Stand

Basic Information

Name:	Single Plane Illumination Microscope (LZ1 - Zeiss Lightsheet Z.1)
Manufacturer:	Zeiss
Model:	LightSheet LZ1 Stand
Facility:	Light Microscopy (LMF)
Partner:	Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG)
Inventory number:	MOZG04089700000

Overview: The lightsheet fluorescence microscopy (also known as SPIM) is a revolutionary development in microscopy. The laser beam illuminates the sample from the side and excites fluorophores along a single line inside of the specimen. A pair of laser scanners moves the excitation laser line vertically and horizontally. An optically sectioned image is recorded by rapidly scanning an entire plane in the specimen and detecting the fluorescence at a right angle to the illumination axis. This technique reduces photobleaching and phototoxic effects in live samples, allowing for long-term imaging in real time in three and four dimensions."The lightsheet fluorescence microscopy (also known as SPIM) is a revolutionary development in microscopy. The laser beam illuminates the sample from the side and excites fluorophores along a single line inside of the specimen. A pair of laser scanners moves the excitation laser line vertically and horizontally. An optically sectioned image is recorded by rapidly scanning an entire plane in the specimen and detecting the fluorescence at a right angle to the illumination axis. This technique reduces photobleaching and phototoxic effects in live samples, allowing for long-term imaging in real time in three and four dimensions." cannot be used as a page name in this wiki.; Suitable for Live imaging in 3D and 4D

Features: 3D stand; -; The system is equipped with a telescopic lens; which allows to zoom out up to 0.36x; and to zoom in up to 2.5x with any detection lens.

Objectives: Zeiss Plan Apochromat 20x 1.0 W DIC; Zeiss Plan Apochromat 20x 1.0 W DIC; Zeiss EC Plan Neofluar 5x 0.16; Zeiss Plan Apochromat 40x 1.0 W DIC; Zeiss Plan Apochromat 63x 1.0 W; Zeiss Illumination Optics Lightsheet 10x 0.2; Zeiss Illumination Optics Lightsheet 10x 0.2; Zeiss Illumination Optics Lightsheet 5x 0.1; Zeiss Illumination Optics Lightsheet 5x 0.1; Zeiss Illumination Optics Lightsheet 10x 0.2

Illumination: Laser Diode 405 nm; Laser DPSS 488 nm; Laser DPSS 561 nm; Laser DPSS 639nm; Transmitted Light (LED)

Reflectors: Laser blockers:
405/488/561
405/488/561/640
405/488/640
ND4 (RGB); Secondary beam splitters and associated emission filters:
LP 490 coupled to SP 490; and LP505
LP 510 coupled to BP 420-470; and BP 525-565
LP 560 coupled to BP 505-545; and BP 575-615
LP 580 coupled to BP 525-565; and LP 585
LP 640 coupled to BP 575-615; and LP 660