Multiphoton Laser Scanning Microscope (ML1 - Leica 2photon in-vivo) Leica DMI6000

Basic Information

Name:	Multiphoton Laser Scanning Microscope (ML1 - Leica 2photon in-vivo)
Manufacturer:	Leica
Model:	DMI6000
Facility:	Light Microscopy (LMF)
Partner:	Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG)

Overview: Multiphoton Laser Scanning Microscope equipped with an 8kHz resonant / galvo tandem scanner. Multiphoton excitation is possible by two pulsed NIR laser lines: one tunable from 680nm-1300nm, one fixed at 1040nm, and detected by four non-descanned detectors (two HyDs and two PMTs). It can also be used as a single photon confocal system with five spectral detectors (four HyDs and one PMT)."Multiphoton Laser Scanning Microscope equipped with an 8kHz resonant / galvo tandem scanner. Multiphoton excitation is possible by two pulsed NIR laser lines: one tunable from 680nm-1300nm, one fixed at 1040nm, and detected by four non-descanned detectors (two HyDs and two PMTs). It can also be used as a single photon confocal system with five spectral detectors (four HyDs and one PMT)." cannot be used as a page name in this wiki.; Suitable for intravital / in vivo imaging and thick specimens. Fast imaging of sensitive samples, spectral imaging, tile scan and multi-position scan.

Features: Inverted stand; XY motorized; motorized Z-focus; Super-Z Galvo Table; resonant scanner; hybrid detectors; simultaneous imaging; sequential imaging; line scan; fast point scan; fast Z stacks; time-series; scan zoom and rotation; tile scan; multi position scan; spectral scan

Objectives: Leica HC CS PL APO 10x 0.4; Leica HC CS PL APO 20x 0.7; Leica HC CS2 PL APO 20x 0.75 Imm; Leica HCX IRAPO L 25x 0.95 W; Leica HC PL IRAPO 40x 1.1 W; Leica HC CS2 PL APO 63x 1.3 Glyc 23/37°C

Illumination: Laser Diode 405 nm; Laser Solid State 488 nm; Laser Solid State 552 nm; Laser Solid State 638 nm; Laser Multiphoton Spectra Physics InSight Deep See Dual; Fluorescence (Metal Halide)

Detection: Confocal: spectral detector with five detectors: four hybrid detectors and one PMT; Multiphoton: four non-descanned detectors: two hybrid detectors and two PMTs