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Structured Illumination Microscope (Zeiss ApoTome - DZNE W1) Zeiss Axio Imager.M2

Basic Information

Name: Structured Illumination Microscope (Zeiss ApoTome - DZNE W1)
Manufacturer: Zeiss
Model: Axio Imager.M2
Facility: Light Microscopy Facility (LMF)
Partner: German Center for Neurodegenerative Diseases Within The Helmholtz Association (DZNE)

Description

Category
Light Microscope
Overview
It is designed for routine work on fixed samples. Optical sectioning can be obtained on fixed samples by the use of the Apotome.
Suitable for fixed samples, fluorescently labeled samples (structured illumination possible), histologically fixed samples (no strucutred illumination), big samples
Features
Upright stand
motorized XY stage (Märzhäuser SMC2009)
motorized z-drive
fluorescence
transmitted light with DIC
Darkfield b/w epifluorescence acquisition
Optical Sectioning (ApoTome2)
sequential imaging
Brightfield and Fluorescence overlay
timelapse
z-stack
tile scans
multi position experiments
complex experiments via Experiment Designer
optical sectioning
Objectives
Zeiss EC Plan Neofluar 5x 0.16
Zeiss Plan-Apochromat 10x 0.45
Zeiss Plan-Apochromat 20x 0.8
Zeiss Plan-Apochromat 40x 0,95 korr.
Zeiss EC Plan-Neofluar 100x 1.3 Oil
Illumination
Fluorescence (Metal Halide, HXP, 120W)
Transmitted Light (Halogen)
Detection

  • Axiocam MRm rev.3 (1388*1040
6,45*6,45µm)
Reflectors

  • FS 49 (DAPI)  : EX BP G365
BS 395
EM BP 445/50
FS 38 HE (GFP)  : EX BP 470/40
BS 495
EM BP 525/50
FS 20 (Rhodamine) : EX BP 546/12
BS 560
BP 575-640
FS 50 (Cy5)  : EX BP 640/30 
BS 660 
EM 690/50

  • FS Analysator for DIC and Transmission
Software
ZEN Blue 2012
Incubation
Not available

Link to Further Details

https://www.biodip.de/wiki/Zeiss_ApoTome_-_DZNE_W1

Points of Contact

Mrs. Silke White (Facility Leader)
Email:
Phone:
+49 (0)351 / 210463-716

Images

Last Update

Last updated at: 8 April 2018 at 03:11:23