The FACS Facility provides instrumentation, education and expertise for all your flow cytometry needs. The flow cytometry services include both, multi-parameter analysis and sorting of cell populations based on fluorescence labels and on phenotypic and biochemical properties of the cells.

Services:

• Expertise to help users with experimental design and protocol development
• Analysis and sorting of cell lines and primary tissue cells from various model organisms
• User training on the Calibur Analyzer instrument

Analysis:

• Multi-parameter analysis of cell suspensions (up to 20 parameters with 5 lasers (375, 405, 488, 561, 633nm)
• Cell cycle analysis
• Cell viability and apoptosis
• Analysis of fluorescently labeled cell lines and antibody stainings

Cell sorting:

• Sterile cell sorting of up to 4 subpopulations simultaneously
• Sterile single-cell sorting into multiwell-plates (24, 48, 96 and 384 wells)