DRESDEN-concept Genome Center (DGC)Parent Units:
Technische Universität Dresden (TUD)
Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG)
|phone:||+49 (0) 351 458 82 351|
|address:||Technische Universität Dresden (TUD) / Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG), DRESDEN-concept Genome Center (DGC), CMCB TU Dresden, Fetscherstrasse 105, 01307 Dresden, Germany|
|partner:||Technische Universität Dresden|
|partner:||Max Planck Institute of Molecular Cell Biology and Genetics|
The Dresden Genome Center (DGC) constitutes a shared technology resource that provides the infrastructure for a broad range of state-of-the-art genomic technologies. Different existing facilities from partner institutions covering DNA sequencing / Tilling, Microarrays, NextGen Sequencing and Recombineering (planned) form the units of the DGC, which are available to researchers in the area of life sciences on the Dresden campus.
The Dresden Genome Center (DGC) is an integral part of the DRESDEN-concept. The fundamental aim of the DRESDEN-concept is to further improve the research landscape in Dresden. A major tool to drive improvement is to make available top-level infrastructure for research, which allows researchers to realize their full potential. In the case of the DGC, this is achieved by enabling shared access to sophisticated and expensive technologies in the field of genomic research.
The DGC provides a top level environment for cutting edge research and services focusing on DNA sequencing, microarray-based technologies, mutant generation, and recombineering strategies.
State-of-the-art technologies are available to the entire research community on the Dresden Campus and support biomedical sciences, translational, and basic research.
Efficient usage of expensive infrastructure, such as Next Generation Sequencing devices resulting in lower costs for local users.
The DGC centralizes established high-level expertise in the area of genomic research, which enables high-quality project design and performance as well as innovative technology development.
Interdisciplinary experimental design and processing in a centralized facility support novel and efficient start-to-end approaches for genomic research. The structure of the center is set up as an open extendable framework. New technologies can easily be implemented as required by the Dresden research community.
The heart of the Dresden Genome Center is a centralized high-level infrastructure for Genomic research on the Dresden Campus that provides that provides high-level expertise, as well as shared state-of-the-art technology platforms. The DGC consists of the following three units: Nex Generation Sequencing, Sanger Sequencing and TILLING, Microarray-based technologies.
Under construction - Current Phase II: According to a new legally valid operational model central administrative rules are defined for shared services and infrastructure. Free capacities of the individual service units are available to all partners without administrative restrictions leading to an optimal utilization of available resources.
Next Generation Sequencing
The Deep Sequencing Group (SFB 655/BIOTEC) provides massively parallelized short read sequencing. Standard workflows are available for RNA sequencing, ChIP sequencing and genomic targeted re-sequencing including library preparation, sequencing and bioinformatic data analysis. For customized applications support is given for the experimental design, project processing and data analysis.
Sanger Sequencing and TILLING
The DNA sequencing service provides high-quality Sanger sequencing of various templates on a routine low-scale (single-tube) and large-scale (96- and 384-well plates) basis. We provide quick turnaround times, and professional support to guarantee excellent data quality. The TILLING (Targeting Induced Local Lesions IN Genomes) project was initiated as collaborative effort of several research groups in the MPI CBG and the Biotec to provide a tool to screen for point mutations or sequence variants in any gene of interest. This technology is based on large-scale amplification of a gene fragment of interest by PCR from libraries of mutagenized individuals and subsequent Sanger sequencing to detect sequence variants. TILLING libraries are available for various model organisms such as the zebrafish D. rerio, the fruitfly D. melanogaster, and the worm C. elegans. Modified TILLING approaches have been established to detect sequence variants naturally that occur in plant species such as the aspen.
The Microarray Facility currently provides service for three different commercially available DNA microarray platforms: Agilent, Affymetrix and NimbleGen. The Agilent hybridization oven and microarray confocal laser scanner set-up of the Microarray Facility allows for analysis of other 1x3” glass slide array platforms, also. Such platforms include but are not limited to, GE Healthcare, Illumina, or custom printed arrays. Any type of microarray, e.g. protein, peptide, antibody, lipid, glycan, etc. can be processed in the Microarray Facility. We have expertise in designing custom microarrays, for example DNA Microarrays with the Agilent database eArray. We offer full service of microarray experiments including sample quality control, experimental design, execution of the experiments, data analysis and data storage. For the validation of DNA microarray results, we are equipped and experienced with qPCR analysis.
still to come...
Next Generation Sequencing:
Dr. Andreas Dahl
Biotechnology Center TU Dresden
Deep Sequencing Group SFB655
DNA Sequencing and TILLING service:
Dr. Sylke Winkler
Max Planck Institute of Molecular Cell Biology and Genetics
- DNA Sequencing / TILLING / Genotyping –
Phone: +49 (0) 351 210-2828
Fax: +49 (0) 351 210-1689
Max Planck Institute of Molecular Cell Biology and Genetics
- Microarray Analysis -
Phone: +49 (0) 351 210-1836
Fax: +49 (0) 351 210-1609
|Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG)||Institute|
|Technische Universität Dresden (TUD)||University|
Last updated at: 2018-11-15 16:13 CET