Superresolution Microscope (H2 - Nikon STORM - TIRF) Nikon Eclipse Ti
Informationen
Name: | Superresolution Microscope (H2 - Nikon STORM - TIRF) | |
Hersteller: | Nikon | |
Modell: | Eclipse Ti | |
Einrichtung: | Abteilung für Lichtmikroskopie (LMF) | |
Partner: | Max-Planck-Institut für Molekulare Zellbiologie und Genetik (MPI-CBG) | |
Inventarnummer: | 0 10.1.32.156 |
Beschreibung
- Category
- Light Microscope
- Overview
- Nikon TIRF / N-STORM setup with two EM-CCDs for detection: at right port with a Hamamatsu W-View image splitter for simultaneous dual-color imaging; at left port with cylindrical lens for 3D-STORM; continuous laser-based autofocus PFS; strong 647nm (300mW), 561nm (150mW), and 488nm (300mW) lasers;"Nikon TIRF / N-STORM setup with two EM-CCDs for detection: at right port with a Hamamatsu W-View image splitter for simultaneous dual-color imaging; at left port with cylindrical lens for 3D-STORM; continuous laser-based autofocus PFS; strong 647nm (300mW), 561nm (150mW), and 488nm (300mW) lasers;" cannot be used as a page name in this wiki.
- Suitable for N-STORM, fast dual-color TIRF, long-term focus-stabilized low-light level imaging
- Features
- Inverted stand
- motorized stage
- double-floor with two reflector-revolvers
- manual 1.5x zoom lens;motorized TIRF-condenser with additional manual collimating lens
- Objectives
- Nikon Plan-Apochromat 10x 0.45 DIC
- Nikon Plan-ApochromatVC 60x 1.2 Water DIC
- Nikon Apochromat 60x 1.49 Oil DIC
- Nikon ApochromatVC 100x 1.4 Oil DIC
- Nikon SR-Apochromat 100x 1.49 Oil DIC
- Nikon Planfluor 20x/0.75 MImm
- Illumination
- Transmitted Light (Halogen)
- Fluorescence (LED)
- Laser Diode 405 nm
- Laser DPSS 488 nm
- Laser DPSS 561 nm
- Laser DPSS 640 nm
- Detection
- at left camera port: Andor iXon EM+ DU-897 BV back-illuminated EM-CCD SN #4431 with cylindrical lens
- dexel size of camera chip: 16um
at right camera port: Andor iXon3 DU-897 BV back-illuminated EM-CCD # 6499 with 10 position filter wheel and Hamamtsu W-View image splitter - dexel size of camera chip: 16um
- Reflectors
- Microscope stand:
reflector revolver FL2 (lower floor):
position 1: L405 cube (EX: BL 406/15 - DC: 425DCXR
- EM: BL HC 457/50)
- 2: L470 cube (EX: ET 470/40
- DC: 495 LP
- EM: ET 525/30)
- 3: Dual LGFP/mCherry cube (EX: dual-band 475/30 + 570/30
- DC: dual-band TM GFP/mCherry
- EM dual-band 520/40 + 630/60)
- 4: narrowGFP (only EM 510/40)
- 5: L620 cube (EX: ET BP 620/60
- DC: 660 LPXR
- EM: BP 700/75)
- 6: empty
reflector revolver FL1 (upper floor):
position 1: TIRF1 cube (quad DC: zt405/488/561/640rpc + quad EM: 446/523/600/677 with 4 TM bands: 446/34 + 523/42 + 600/36 + 677/28) - 2: TIRF2Q cube (quad DC: zt 405/488/561/640
- quad EM: 446/523/600/677 with 4 TM bands: 446/34 + 523/42 + 600/36 + 677/28)
- 3: HC YFP cube (EX: BL HC 500/24
- DC: HC BS 520LP
- EM: BL HC 542/27)
- 4: L565 cube (EX: BL HC 562/40
- DC: BS 593
- EM: BL HC 624/40)
- 5: Analyzer
- 6: empty
Sutter filter wheel:
position 0: BrightLine 617/73 - 1: Razor Edge LP488RS
- 2: Razor Edge LP664RS
- 3: BrightLine 447/60
- 4: open
- 5: Dual BrightLine 512/630 (512/23 + 630/91)
- 6: BrightLine 628/40
- 7: BrightLine 525/30
- 8: BrightLine 607/36
- 9: BrightLine 520/15
Hamamatsu W-View image splitter:
for bypass mode: silver mirror
for split mode: dichromatic mirror pair: 560 DCSP (transmission 500/100 - reflection 615/90) and 560 DCLP (reflection 500/100
- transmission 635/130)
- emission filters: ET BP 535/30
- BL HC 628/40
- Software
- NIS Elements 4.51.01 with N-STORM module + Jobs module
- Incubation
- Cage incubator (T+CO2)
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Letztes Update
Zuletzt aktualisiert am: 8. April 2018 um 03:10:49