Superresolution Microscope (H2 - Nikon STORM - TIRF) Nikon Eclipse Ti

Informationen

Name:	Superresolution Microscope (H2 - Nikon STORM - TIRF)
Hersteller:	Nikon
Modell:	Eclipse Ti
Einrichtung:	Abteilung für Lichtmikroskopie (LMF)
Partner:	Max-Planck-Institut für Molekulare Zellbiologie und Genetik (MPI-CBG)
Inventarnummer:	0 10.1.32.156

Overview: Nikon TIRF / N-STORM setup with two EM-CCDs for detection: at right port with a Hamamatsu W-View image splitter for simultaneous dual-color imaging; at left port with cylindrical lens for 3D-STORM; continuous laser-based autofocus PFS; strong 647nm (300mW), 561nm (150mW), and 488nm (300mW) lasers;"Nikon TIRF / N-STORM setup with two EM-CCDs for detection: at right port with a Hamamatsu W-View image splitter for simultaneous dual-color imaging; at left port with cylindrical lens for 3D-STORM; continuous laser-based autofocus PFS; strong 647nm (300mW), 561nm (150mW), and 488nm (300mW) lasers;" cannot be used as a page name in this wiki.; Suitable for N-STORM, fast dual-color TIRF, long-term focus-stabilized low-light level imaging

Features: Inverted stand; motorized stage; double-floor with two reflector-revolvers; manual 1.5x zoom lens;motorized TIRF-condenser with additional manual collimating lens

Objectives: Nikon Plan-Apochromat 10x 0.45 DIC; Nikon Plan-ApochromatVC 60x 1.2 Water DIC; Nikon Apochromat 60x 1.49 Oil DIC; Nikon ApochromatVC 100x 1.4 Oil DIC; Nikon SR-Apochromat 100x 1.49 Oil DIC; Nikon Planfluor 20x/0.75 MImm

Illumination: Transmitted Light (Halogen); Fluorescence (LED); Laser Diode 405 nm; Laser DPSS 488 nm; Laser DPSS 561 nm; Laser DPSS 640 nm

Detection: at left camera port: Andor iXon EM+ DU-897 BV back-illuminated EM-CCD SN #4431 with cylindrical lens; dexel size of camera chip: 16um
at right camera port: Andor iXon3 DU-897 BV back-illuminated EM-CCD # 6499 with 10 position filter wheel and Hamamtsu W-View image splitter; dexel size of camera chip: 16um

Reflectors: Microscope stand:
reflector revolver FL2 (lower floor):
position 1: L405 cube (EX: BL 406/15; DC: 425DCXR; EM: BL HC 457/50); 2: L470 cube (EX: ET 470/40; DC: 495 LP; EM: ET 525/30); 3: Dual LGFP/mCherry cube (EX: dual-band 475/30 + 570/30; DC: dual-band TM GFP/mCherry; EM dual-band 520/40 + 630/60); 4: narrowGFP (only EM 510/40); 5: L620 cube (EX: ET BP 620/60; DC: 660 LPXR; EM: BP 700/75); 6: empty
reflector revolver FL1 (upper floor):
position 1: TIRF1 cube (quad DC: zt405/488/561/640rpc + quad EM: 446/523/600/677 with 4 TM bands: 446/34 + 523/42 + 600/36 + 677/28); 2: TIRF2Q cube (quad DC: zt 405/488/561/640; quad EM: 446/523/600/677 with 4 TM bands: 446/34 + 523/42 + 600/36 + 677/28); 3: HC YFP cube (EX: BL HC 500/24; DC: HC BS 520LP; EM: BL HC 542/27); 4: L565 cube (EX: BL HC 562/40; DC: BS 593; EM: BL HC 624/40); 5: Analyzer; 6: empty
Sutter filter wheel:
position 0: BrightLine 617/73; 1: Razor Edge LP488RS; 2: Razor Edge LP664RS; 3: BrightLine 447/60; 4: open; 5: Dual BrightLine 512/630 (512/23 + 630/91); 6: BrightLine 628/40; 7: BrightLine 525/30; 8: BrightLine 607/36; 9: BrightLine 520/15
Hamamatsu W-View image splitter:
for bypass mode: silver mirror
for split mode: dichromatic mirror pair: 560 DCSP (transmission 500/100; reflection 615/90) and 560 DCLP (reflection 500/100; transmission 635/130); emission filters: ET BP 535/30; BL HC 628/40

LMF

Web:

E-Mail:

Telefon:

+49 351 210-2345

Fax:

+49 351 210-1078

Vorschau	Beschreibung
	H2 - Nikon STORM - TIRF.jpg

Zuletzt aktualisiert am: 8. April 2018 um 03:10:49