Alle Geräte dieser Einrichtung anzeigen Real-Time Confocal Microscope (SD2 - Andor Spinning Disc FRAPPA) Olympus IX81
Informationen
| Name: | Real-Time Confocal Microscope (SD2 - Andor Spinning Disc FRAPPA) | |
| Hersteller: | Olympus | |
| Modell: | IX81 | |
| Einrichtung: | Abteilung für Lichtmikroskopie (LMF) |
|
| Partner: | Max-Planck-Institut für Molekulare Zellbiologie und Genetik (MPI-CBG) |
|
| Inventarnummer: | 0 10.1.145.20 | |
Beschreibung
- Category
- Light Microscope
- Overview
- Fast, "real-time" confocal imaging with FRAPPA capability. Illumination equipment: Cairn OptoLED lite, 405nm diode laser (100mW) + 488 nm solid-state laser Coherent Sapphire (50mW) + 561 nm solid-state laser Cobolt Jive (50mW) + 640 nm solid-state laser (100mW)."Fast, "real-time" confocal imaging with FRAPPA capability. Illumination equipment: Cairn OptoLED lite, 405nm diode laser (100mW) + 488 nm solid-state laser Coherent Sapphire (50mW) + 561 nm solid-state laser Cobolt Jive (50mW) + 640 nm solid-state laser (100mW)." cannot be used as a page name in this wiki.
- Suitable for confocal imaging, photobleaching and -activation of DAPI,EGFP, mCherry, Cy5 with high temporal and spatial resolution
- Features
- Inverted stand
- motorized with ZDC (hardware autofocus)
- 2x Optovar
- Prior NanoScanZ
- Prior ProScanIII xy scanning stage
- objectives 40x 1.25 SIL and 60x 1.3 SIL are shared objectives and need to be booked separately via the LMF booking database!
- Objectives
- Olympus UPlanSApochromat 10x 0.4
- Olympus UPlanSApochromat 60x 1.2 W
- Olympus UPlanSApochromat 60x 1.35 Oil
- Olympus UPlanSApochromat 100x 1.4 Oil
- Olympus UApochromat 40x 1.15 W
- Illumination
- Transmitted Light (LED)
- Fluorescence (HBO)
- Laser Diode 405 nm
- Laser DPSS 488 nm
- Laser DPSS 561 nm
- Laser DPSS 640 nm
- Detection
- [[detection::Spinning disc scan head Yokogawa CSU-X1 (5000rpm):
dichromatic mirrors:
Quad band T-405/488/568/647
Triple band T-405/488/561
Single band T-488
physical pinhole radius: 25um - pinhole spacing: 250um
- back-projected pinhole size: 0.4um
- back-projected pinhole spacing: 4um (with 60x objective
- 1x optovar)
Andor Dual camera port with two cameras:
Andor iXon EM+ DU-897 BV back illuminated EMCCD - dexel size of EMCCD chip: 16um
Andor Clara cooled interline CCD - dexel size of chip: 6.45um
note there are two additional lenses in the optical path: 1.0x before the CSU and another one after the CSU: a 1.97x in front of iXon or a 0.95x in front of Clara
image pixel size for use with iXon (measured Sept - 8th 2012): with 100xoil objective: 0.081um/px (optovar 1x)
- 0.041um/px (opt
- 2x)
- with 60xW obj.: 0.136um/px (opt.1x)
- 0.068um/px (opt 2x)
- 10x: 0.811um/px (opt.1x)
- 0.407um/px (opt.2x)
pixel size with Clara (measured Sept - 8th 2012): 100x oil objective: 0.068um/px (opt 1x)
- 0.034um/px (opt2x)
- 60xW obj.: 0.114um/px (opt.1x)
- 0.057um/px (opt
- 2x)
- 10x obj: 0.68um/px (opt
- 1x)
- 0.34um/px (opt
- 2x)
old values:
projecting lens in front of iXon: was 2.475 before installation of TuCAM on Sept 4th 2012 - was 2.39x before removal of DualView on Dec 21st 2011
- was 2.067x before addition of the dual filter wheel on Apr 28th 2011
- was 1.9x before addition of dual camera switch on June 30th 2010
projecting lens in front of Clara: was 1.285x before installation of TuCAM on Sept 4th 2012 - was 1.28x before removal of DualView on Dec 21st 2011
- was 1.2x before addition of the dual filter wheel on Apr 28th 2011
image pixel size for use with iXon (measured Jan 2012): with 100xoil objective: 0.0646um/px (optovar 1x) - 0.0322um/px (opt
- 2x)
- with 60xW obj.: 0.108um/px (opt.1x)
- 0.054um/px (opt 2x)
- 60x oil obj.: 0.1074um/px (opt
- 1x)
- 0.0537um/px (opt
- 2x)
- 10x: 0.648um/px (opt.1x)
- 0.324um/px (opt.2x)
pixel size with Clara (measured Jan 2012): 100x oil objective: 0.05um/px (opt 1x) - 0.025um/px (opt2x)
- 60xW obj.: 0.084um/px (opt.1x)
- 0.042um/px (opt
- 2x)
- 60xoil obj.: 0.0834um/px (opt.1x)
- 0.0417um/px (opt
- 2x)
- 10x obj: 0.502um/px (opt
- 1x)
- 0.251um/px (opt
- 2x)
image pixel size used with iXon (measured Ju;y 15th 2011): with 100x: 0.067(was 0.077um before Apr 2011) - with 60x objective: 0.111 (was 0.129 before Apr 2011) (was 0.14um)
- 60x obj
- and 2.0x optovar: was 0.0645um (was 0.07um)
- 40x obj.: was 0.194um (was 0.21um)
- 40x obj
- and 2.0x opt.: was 0.0967um (was 0.11um)
pixel size with Clara (measured July 15th 2011 - all with optovar 1x): 100x objective: 0.05um/px
- 60x obj.: 0.084um/px
- 10x obj: 0.511um/px
]]
- Reflectors
- Sutter filter wheel A:
position 0: 440/521/607/700 Quadband - 1: razor edge 488LP
- 2: BL700/75
- 3: razor edge 568LP
- 4: Edge Basic longpass 635 LP
- 5: BrightLine 445/45
- 6: open
- 7: polarizer for DIC
- 8: 525/30
- 9: BrightLine HC 607/36
Sutter filter wheel B:
position 0: empty/open - position 1: BL 470/22
- positions 2 - 9: empty/open
microscope filter turret:
position 1: empty - 2: DIC
- 3: tripple DAPI + GFP + RFP cube
- 4: GFP
- 5: RFP
- 6: DAPI cube (X 345/10
- D 380LP
- M 445/40)
- Software
- [[software::iQ 3.5 (with Python) iQ3 user guide
- connections: COM1 - stage
- COM2xx - lasers
- COM3 - CSU
- COM4 - Sutter FW
- COM5 - Olympus IX 81
- COM6 - FRAPPA
- AOTF - ALC401/501/601
- DD A06/16]]
- Incubation
- Stage incubator (T) + objective heater
Link zu weiteren Informationen
Ansprechpartner
Bilder
| Vorschau | Beschreibung |
|---|---|
|
2012 09 10 SD2 FRAPPA.jpg |
Letztes Update
Zuletzt aktualisiert am: 8. April 2018 um 03:11:05