Real-Time Confocal Microscope (H3 Andor STORM Spinning disc)

Informationen

Name:	Real-Time Confocal Microscope (H3 Andor STORM Spinning disc)
Einrichtung:	Abteilung für Lichtmikroskopie (LMF)
Partner:	Max-Planck-Institut für Molekulare Zellbiologie und Genetik (MPI-CBG)

Overview: Fast, "real-time" confocal imaging with FRAPPA capability. Illumination equipment: TM-LED, Andor AMH, 405nm diode laser + 488 nm solid-state laser Coherent Sapphire + 561 nm solid-state laser + 640 nm solid-state laser.; Suitable for confocal imaging, photobleaching and -activation of DAPI,EGFP, mCherry, Cy5 with high temporal and spatial resolution

Features: Inverted stand; motorized with PFS (hardware autofocus); 1.5 x Optovar; MCL Piezo 100um travel range; motorized XY stage; FRAPPA coupled in via backport

Objectives: Nikon Plan-Apochromat 10x 0.45 DIC; Nikon Plan-Fluor 20x 0.75 Oil/Glyc/Water DIC; Nikon Plan-Apochromat 60x 1.2 W; Nikon Apo 100x/1.49 Oil DIC 0.13-0.20

Illumination: Transmitted Light (LED); Fluorescence (Metal Halide); Laser Diode 405 nm; Laser DPSS 488 nm; Laser DPSS 561 nm; Laser DPSS 640 nm

Detection: [[detection::Spinning disc scan head Yokogawa CSU-X1 (10000rpm); manual:
dichromatic mirror:
Quad band T-405/488/568/647
physical pinhole radius: 25um; pinhole spacing: 250um; back-projected pinhole size: 0.4um; back-projected pinhole spacing: 4um (with 60x objective; 1x optovar)
camera:
Andor iXon EM+ DU-897 BV back illuminated EMCCD; dexel size of EMCCD chip: 16um; image pixel sizes:
with 10x air obj.: 1.048um/px (1x optovar); 0.699 um/px (1.5x opt.); with 20x Oil/Glyc/Water obj.: 0.524um/px (1x optovar); 0.349 um/px (1.5x opt.); with 60x W obj.: 0.175um/px (1x opt.); 0.116um/px (1.5x opt.); with 100x oil obj.: 0.105um/px (1x opt.); 0.0698um/px (1.5x opt.)]]

Reflectors: Rotr filter wheel:
position 0: 520/15; 1: 628/40; 2: 447/60; 3: 607/36; 4: dual 512/630; 5: LP 664 RU; 6: 525/30; 7: 716/40; 8: LP 488 razor edge; 9: 617/73

Software: [[software::iQ 2.9 + iQ 3.4.1 (both with Python) iQ3 user guide; connections: COM1 - ????; COM2xx - lasers; COM3 - ???; COM4 - ???; COM5 - ???; COM6 - ???; AOTF - ALC401/501/601; DD A06/16]]

LMF

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+49 351 210-2345

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+49 351 210-1078

Zuletzt aktualisiert am: 8. April 2018 um 03:10:50