View all instruments of this unit Laser Scanning Confocal Microscope (CO1 - Olympus Fluoview 1000) Olympus BX 61
Basic Information
| Name: | Laser Scanning Confocal Microscope (CO1 - Olympus Fluoview 1000) | |
| Manufacturer: | Olympus | |
| Model: | BX 61 | |
| Facility: | Light Microscopy (LMF) |
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| Partner: | Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG) |
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| Inventory number: | MOZG0407421000 10.1.38.205 | |
Description
- Category
- Light Microscope
- Overview
- [[description::Single photon point scanning Olympus FV1000 confocal microscope with four confocal PMTs and one transmitted light detector. Inverted configuration with additional scanner and pulsed picosecond laser]]
- Suitable for ablation experiments
- Features
- Inverted stand
- motorized XY stage
- motorized Z-drive
- fluorescence
- transmitted light with manual DIC
- Objectives 40x 1.25 SIL and 60x 1.3 SIL are shared objectives and need to be booked separately via the LMF booking database!
- Two independent and fully synchronized laser scanners for simultaneous laser stimulation and observation
- laser stimulation can be performed using the imaging laser or a 405nm picosecond Diode laser (PicoQuant)
- sequential imaging
- line scan
- point scan
- Z stacks
- time-series
- advanced time-series
- Kalman averaging
- summarizing
- scan zoom and rotation
- advanced use of motorized stage due to MATL-software
- which allows independent multi-point acquisition and tiling + some simple stitching
- Objectives
- Olympus UPlanSApochromat 10x 0.4
- Olympus UPlanSApochromat 40x 0.9
- Olympus UPlanSApochromat 20x 0.85 Oil
- Olympus UApochromat 40x 1.35 Oil
- Olympus UPlanSApochromat 60x 1.35 Oil
- Olympus UPlanApochromat 60x 1.2 W
- Illumination
- Transmitted Light (Halogen)
- Fluorescence (Metal Halide, X-Cite, 120W)
- Laser Diode 405 nm
- Laser Argon Multiline 458, 477, 488, 514 nm
- Laser DPSS 561 nm
- Laser HeNe 633 nm
- Laser Diode 405 nm (pulsed)
- Detection
- [[detection::point scanner
- 4 confocal PMTs
- two independent spectral detection channels
- each one configured with a diffraction grating and variable slit for high-resolution wavelength separation and high-speed bandwidth selection
- two filter based detection channels
- T-PMT
- dichromatic mirrors for excitation/emission splitting
- one pinhole]]
- Reflectors
- [[reflectors::Filter Cube 1
- U-MWU2
- BP330-385 BA420 DM400
- Filter Cube 2
- U-MWB2
- BP460-490 BA520IF (High performance interference filter) DM500
Filter Cube 3 - U-MWG2
- BP510-550 BA590 DM570]]
- Software
- FV10-ASW 1.7
- Incubation
- Stage incubator (T+CO2)
Link to Further Details
Points of Contact
Images
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Last Update
Last updated at: 8 April 2018 at 03:10:46