Laser Scanning Confocal Microscope (CO1 - Olympus Fluoview 1000) Olympus BX 61

Informationen

Name:	Laser Scanning Confocal Microscope (CO1 - Olympus Fluoview 1000)
Hersteller:	Olympus
Modell:	BX 61
Einrichtung:	Abteilung für Lichtmikroskopie (LMF)
Partner:	Max-Planck-Institut für Molekulare Zellbiologie und Genetik (MPI-CBG)
Inventarnummer:	MOZG0407421000 10.1.38.205

Overview: [[description::Single photon point scanning Olympus FV1000 confocal microscope with four confocal PMTs and one transmitted light detector. Inverted configuration with additional scanner and pulsed picosecond laser]]; Suitable for ablation experiments

Features: Inverted stand; motorized XY stage; motorized Z-drive; fluorescence; transmitted light with manual DIC; Objectives 40x 1.25 SIL and 60x 1.3 SIL are shared objectives and need to be booked separately via the LMF booking database!; Two independent and fully synchronized laser scanners for simultaneous laser stimulation and observation; laser stimulation can be performed using the imaging laser or a 405nm picosecond Diode laser (PicoQuant); sequential imaging; line scan; point scan; Z stacks; time-series; advanced time-series; Kalman averaging; summarizing; scan zoom and rotation; advanced use of motorized stage due to MATL-software; which allows independent multi-point acquisition and tiling + some simple stitching

Objectives: Olympus UPlanSApochromat 10x 0.4; Olympus UPlanSApochromat 40x 0.9; Olympus UPlanSApochromat 20x 0.85 Oil; Olympus UApochromat 40x 1.35 Oil; Olympus UPlanSApochromat 60x 1.35 Oil; Olympus UPlanApochromat 60x 1.2 W

Illumination: Transmitted Light (Halogen); Fluorescence (Metal Halide, X-Cite, 120W); Laser Diode 405 nm; Laser Argon Multiline 458, 477, 488, 514 nm; Laser DPSS 561 nm; Laser HeNe 633 nm; Laser Diode 405 nm (pulsed)

Detection: [[detection::point scanner; 4 confocal PMTs; two independent spectral detection channels; each one configured with a diffraction grating and variable slit for high-resolution wavelength separation and high-speed bandwidth selection; two filter based detection channels; T-PMT; dichromatic mirrors for excitation/emission splitting; one pinhole]]

Reflectors: [[reflectors::Filter Cube 1; U-MWU2; BP330-385 BA420 DM400; Filter Cube 2; U-MWB2; BP460-490 BA520IF (High performance interference filter) DM500
Filter Cube 3; U-MWG2; BP510-550 BA590 DM570]]

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Zuletzt aktualisiert am: 8. April 2018 um 03:10:46