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Total Internal Reflection Microscope (Live Cell/TIRF AF6000, BIOTEC) Leica DMI6000

Basic Information

Name: Total Internal Reflection Microscope (Live Cell/TIRF AF6000, BIOTEC)
Manufacturer: Leica
Model: DMI6000
Facility: Light Microscopy (LMF)
Partner: Technische Universität Dresden (TUD)
Inventory number: 71925

Description

Category
Light Microscope
Overview
Our Leica AFLX6000 TIRF is designed for observation of LiveCell systems (incubator) and phenomenons at the interface between the cells and their substrate. The laser lines are well distributed over the spectrum and especially good for common dye combinations. A system of external emission filters wheel allows extremely fast acquisition in combination with an EMCCD for detection of low signal."Our Leica AFLX6000 TIRF is designed for observation of LiveCell systems (incubator) and phenomenons at the interface between the cells and their substrate. The laser lines are well distributed over the spectrum and especially good for common dye combinations. A system of external emission filters wheel allows extremely fast acquisition in combination with an EMCCD for detection of low signal." cannot be used as a page name in this wiki.
Features
Inverted stand
motorized XY stage
Super Z-stage
fluorescence
transmitted light with automatic DIC
TIRF
sequential imaging
Z stacks
time-series
advanced time-series
multiple position imaging
fast acquisition (with Andor EMCCD)
Objectives
Leica HC PL APO 10x 0.4
Leica HC PL APO 20x 0.7
Leica HCX PL APO 40x 0.85
Leica HC PL APO 100x 1.46 Oil
Illumination
Laser Diode 405 nm
Laser DPSS 488 nm
Laser DPSS 561 nm
Laser HeNe 633 nm
Fluorescence (Metal Halide, HXP, 120W)
Transmitted Light (Halogen)
Detection
Andor iXON 897 EMCCD (512*512
16µm pixels)
Leica DFC (1392*1040
6,45µm pixels)
reflectors for fluorescence and TIRF
external emission filter wheel for rapid acquisition with Andor EMCCD
Reflectors
FITC (L5) : EX 480/40 
BS 505 
EM 527/30
CY5 : EX 635/10 
BS 650 
EM BP 720/60
Quad Laser Line
VBG : EX 490/20 and 422/44 and 552/24 
BS 435 and 505 and 550 
EM 465/45 and 545/55 and 610/65
RFP : EX 552/24 
BS 570 
EX 605/65
Software
LAS AF
Incubation
Cage incubator (T+CO2)

Link to Further Details

https://www.biodip.de/wiki/Live_Cell/TIRF_AF6000,_BIOTEC

Points of Contact

Mrs. Dr. Hella Hartmann
Email:
Phone:
+49 (0)351 463-40230
Fax:
+49 (0) 351 463 40038

Images

Last Update

Last updated at: 8 April 2018 at 03:10:58