View all instruments of this unit Total Internal Reflection Microscope (Live Cell/TIRF AF6000, BIOTEC) Leica DMI6000
Basic Information
| Name: | Total Internal Reflection Microscope (Live Cell/TIRF AF6000, BIOTEC) | |
| Manufacturer: | Leica | |
| Model: | DMI6000 | |
| Facility: | Light Microscopy (LMF) |
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| Partner: | Technische Universität Dresden (TUD) |
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| Inventory number: | 71925 | |
Description
- Category
- Light Microscope
- Overview
- Our Leica AFLX6000 TIRF is designed for observation of LiveCell systems (incubator) and phenomenons at the interface between the cells and their substrate. The laser lines are well distributed over the spectrum and especially good for common dye combinations. A system of external emission filters wheel allows extremely fast acquisition in combination with an EMCCD for detection of low signal."Our Leica AFLX6000 TIRF is designed for observation of LiveCell systems (incubator) and phenomenons at the interface between the cells and their substrate. The laser lines are well distributed over the spectrum and especially good for common dye combinations. A system of external emission filters wheel allows extremely fast acquisition in combination with an EMCCD for detection of low signal." cannot be used as a page name in this wiki.
- Features
- Inverted stand
- motorized XY stage
- Super Z-stage
- fluorescence
- transmitted light with automatic DIC
- TIRF
- sequential imaging
- Z stacks
- time-series
- advanced time-series
- multiple position imaging
- fast acquisition (with Andor EMCCD)
- Objectives
- Leica HC PL APO 10x 0.4
- Leica HC PL APO 20x 0.7
- Leica HCX PL APO 40x 0.85
- Leica HC PL APO 100x 1.46 Oil
- Illumination
- Laser Diode 405 nm
- Laser DPSS 488 nm
- Laser DPSS 561 nm
- Laser HeNe 633 nm
- Fluorescence (Metal Halide, HXP, 120W)
- Transmitted Light (Halogen)
- Detection
- Andor iXON 897 EMCCD (512*512
- 16µm pixels)
- Leica DFC (1392*1040
- 6,45µm pixels)
- reflectors for fluorescence and TIRF
- external emission filter wheel for rapid acquisition with Andor EMCCD
- Reflectors
- FITC (L5) : EX 480/40
- BS 505
- EM 527/30
CY5 : EX 635/10 - BS 650
- EM BP 720/60
Quad Laser Line
VBG : EX 490/20 and 422/44 and 552/24 - BS 435 and 505 and 550
- EM 465/45 and 545/55 and 610/65
RFP : EX 552/24 - BS 570
- EX 605/65
- Software
- LAS AF
- Incubation
- Cage incubator (T+CO2)
Link to Further Details
Points of Contact
Images
| Preview | Description |
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Bioz06 - Leica AFLX6000 TIRF.jpg |
Last Update
Last updated at: 8 April 2018 at 03:10:58