Laser Scanning Confocal Microscope (SP5 I, upright, BIOTEC) Leica DM6000

Basic Information

Name:	Laser Scanning Confocal Microscope (SP5 I, upright, BIOTEC)
Manufacturer:	Leica
Model:	DM6000
Facility:	Light Microscopy (LMF)
Partner:	Technische Universität Dresden (TUD)
Inventory number:	146330

Overview: [[description::Our Leica TCS SP5 I confocal setup is designed for working on living sample and dedicated for high resolution imaging in XYZ. The upright configuration and the dipping lenses make it optimal for embryo development studies. An incubator allows long timelapse with living samples. The laser lines are well distributed over the spectrum and especially good for common dye combinations. Due to the conventional setup, it's straight forward to work with.]]

Features: upright stand; motorized XYZ stage; Z-piezo stage; fluorescence; transmitted light with automatic DIC; simultaneous imaging; sequential imaging; line scan; point scan; Z stacks; multi-position imaging; time-series; advanced time-series; averaging; summarizing; scan zoom and rotation

Objectives: Leica HCX PL APO 40x 0.75; Leica HC PL APO 20x 0.7; Leica HC PL APO 10x 0.4; Leica HC PL FLUOTAR 5x 0.15; Leica HC PL APO CS 40x 1,25-0,75 Oil; Leica HC PL APO 100x 1.4 Oil; Leica HCX PL APO 100x 1.47 Oil Korr.

Illumination: Transmitted Light (Halogen); Fluorescence (Metal Halide, HXP, 120W); Laser Diode 405 nm; Laser Argon Multiline 458, 477, 488, 496, 514 nm; Laser DPSS 561 nm; Laser HeNe 633 nm

Detection: point scanner; 2 confocal HyD´s; 3 confocal PMTs; T-PMT; AOBS for excitation/emission splitting; SP detector for detection range selection; common pinhole

Reflectors: GFP/FITC (L5) : EX 480/40; BS 505; EM BP 527/30; DAPI (A) : EX 360/40; BS 400; EM LP 425
TRITC/DsRed/RFP : EX 545/30; LP 570; EM BP 620/60
Analysator