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Laser Scanning Confocal Microscope (LSM 780/FLIM, inverse, CRTD) Zeiss Axio Observer

Basic Information

Name: Laser Scanning Confocal Microscope (LSM 780/FLIM, inverse, CRTD)
Manufacturer: Zeiss
Model: Axio Observer
Facility: Light Microscopy (LMF)
Partner: Technische Universität Dresden (TUD)
Inventory number: 84844

Description

Category
Light Microscope
Overview
It is an inverted Laser Scanning Confocal microscope with up to 32 spectral detection channels, 2 PMTs and one transmission channel. It provides FLIM functionality.
Suitable for the detection of multiple fluorescent dyes (but no DAPI), FRAP, FRET, optical sectioning for 3D reconstruction, linear and spectral unmixing of overlapping emission spectra. It can acquire brightfield and DIC images with the transmission detector
Features
Inverted stand
-
spectral scan
FLIM dual channel unit from B&H
Objectives
Zeiss Plan-Apochromat 10x 0.45
Zeiss Plan-Apochromat 20x 0.8
Zeiss C-Apochromat 40x 1.2 W
Zeiss alpha Plan-Apochromat 63x 1.46 Oil
Illumination
Laser Diode 440 nm (pulsed)
Laser Diode 475 nm (pulsed)
Laser Argon Multiline 458, 488, 514 nm
Laser DPSS 561 nm
Laser HeNe 633 nm
Fluorescence (Metal Halide, HXP, 120W)
Transmitted Light (Halogen)
Detection
32 PMT channels (GaAsP)
2 PMTs
transmission detector
Reflectors
Zeiss set 49: DAPI
Zeiss set 38: GFP
Zeiss set 47: CFP
Zeiss set 46: YFP
Zeiss set 43: Cy3
Software
ZEN black 2011
Incubation
Cage incubator (T+CO2+Humidity)

Link to Further Details

https://www.biodip.de/wiki/LSM_780/FLIM,_inverse,_CRTD

Points of Contact

Mrs. Dr. Hella Hartmann
Email:
Phone:
+49 (0)351 463-40230
Fax:
+49 (0) 351 463 40038

Images

Last Update

Last updated at: 8 April 2018 at 03:10:55