Single Plane Illumination microscope (Ultramicroscope, CRTD) Olympus MVX10

Basic Information

Name:	Single Plane Illumination microscope (Ultramicroscope, CRTD)
Manufacturer:	Olympus
Model:	MVX10
Facility:	Light Microscopy (LMF)
Partner:	Technische Universität Dresden (TUD)

Overview: Static light sheet microscope with 2 light sheets. Each sheet is composed out of three individual sheets from different angles to reduce shadowing. The sample can be moved about 1cm3 in xyz. Due to the white light laser/excitation/emission filter wheels a broad range of fluorophores can be used."Static light sheet microscope with 2 light sheets. Each sheet is composed out of three individual sheets from different angles to reduce shadowing. The sample can be moved about 1cm³ in xyz. Due to the white light laser/excitation/emission filter wheels a broad range of fluorophores can be used." cannot be used as a page name in this wiki.; Suitable for 3D multicolor imaging of large transparent specimen

Features: upright stand; motorized stage to move the sample holder in xyz inside the glass cuvette; 2 different dipping caps for the objective available; Two different cuvettes/sample holders/dipping caps are available; one for live imaging in water; one for fixed/cleared samples; There is a correction lens in front of the sCMOS camera for chromatic abberation correction in z direction; The light sheet can be tuned in three dimensions: thickness and width of the sheet; overlap of the 2 sheets from opposite directions.

Detection: Andor Neo sCMOS camera; Pixel 2560 x 2160
Pixel size 6.5 x 6.5 μm²

Reflectors: 7 excitation and emission filter:; DAPI: ex; 405/40 ET; em; 460/50 ET Bandpass
GFP: ex; 470/40 ET; em; 525/50 ET Bandpass
YFP: ex; 500/20 ET; em; 535/30 ET Bandpass
Cy3/DsRed/TRITC: ex; 545/30; em; 610/60 ET Bandpass
mCherry/ TxRed: ex; 560/40; em; 630/75 ET Bandpass
Alexa 610/Alexa 633/E2-Crimson: ex; 605/50; em; 670/50 ET Bandpass
Cy7/Alexa 750/DyLight 750: ex; 710/75; em; 810/90 ET Bandpass