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Single Plane Illumination microscope (Ultramicroscope, CRTD) Olympus MVX10

Basic Information

Name: Single Plane Illumination microscope (Ultramicroscope, CRTD)
Manufacturer: Olympus
Model: MVX10
Facility: Light Microscopy (LMF)
Partner: Technische Universität Dresden (TUD)

Description

Category
Light Microscope
Overview
Static light sheet microscope with 2 light sheets. Each sheet is composed out of three individual sheets from different angles to reduce shadowing. The sample can be moved about 1cm3 in xyz. Due to the white light laser/excitation/emission filter wheels a broad range of fluorophores can be used."Static light sheet microscope with 2 light sheets. Each sheet is composed out of three individual sheets from different angles to reduce shadowing. The sample can be moved about 1cm3 in xyz. Due to the white light laser/excitation/emission filter wheels a broad range of fluorophores can be used." cannot be used as a page name in this wiki.
Suitable for 3D multicolor imaging of large transparent specimen
Features
upright stand
motorized stage to move the sample holder in xyz inside the glass cuvette
2 different dipping caps for the objective available
Two different cuvettes/sample holders/dipping caps are available
one for live imaging in water
one for fixed/cleared samples
There is a correction lens in front of the sCMOS camera for chromatic abberation correction in z direction
The light sheet can be tuned in three dimensions: thickness and width of the sheet
overlap of the 2 sheets from opposite directions.
Objectives
Olympus MVPLAPO 2x/0.5
Illumination
Laser Fianium Supercontinuum SC400-4
Detection
Andor Neo sCMOS camera
Pixel 2560 x 2160
Pixel size 6.5 x 6.5 μm2
Reflectors
7 excitation and emission filter:
DAPI: ex
405/40 ET
em
460/50 ET Bandpass
GFP: ex
470/40 ET
em
525/50 ET Bandpass
YFP: ex
500/20 ET
em
535/30 ET Bandpass
Cy3/DsRed/TRITC: ex
545/30
em
610/60 ET Bandpass
mCherry/ TxRed: ex
560/40
em
630/75 ET Bandpass
Alexa 610/Alexa 633/E2-Crimson: ex
605/50
em
670/50 ET Bandpass
Cy7/Alexa 750/DyLight 750: ex
710/75
em
810/90 ET Bandpass
Software
LaVision BioTec ImSpector Software
Windows 7
64 bit
Incubation
Not available

Link to Further Details

https://www.biodip.de/wiki/Ultramicroscope,_CRTD

Points of Contact

Mrs. Dr. Hella Hartmann
Email:
Phone:
+49 (0)351 463-40230
Fax:
+49 (0) 351 463 40038

Images

Last Update

Last updated at: 8 April 2018 at 03:11:12