View all instruments of this unit Real-Time Confocal Microscope (SD4 - Andor Revolution WD Borealis Mosaic) Olympus IX83
Basic Information
| Name: | Real-Time Confocal Microscope (SD4 - Andor Revolution WD Borealis Mosaic) | |
| Manufacturer: | Olympus | |
| Model: | IX83 | |
| Facility: | Light Microscopy (LMF) |
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| Partner: | Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG) |
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Description
- Category
- Light Microscope
- Overview
- Fast, "real-time" spinning disc confocal imaging with photo-activation capability. Fast imaging of large field of view and large tile scans with even illumination.
- Suitable for confocal imaging, photo-activation using 405 laser with high temporal and spatial resolution; fast imaging of large samples over a long period of time
- Features
- Inverted stand
- motorized with ZDC (hardware autofocus and focus search)
- Prior NanoScanZ (400um travel range)
- Prior ProScanIII xy scanning stage
- Objectives
- Olympus UPlan SApochromat 10x 0.4
- Olympus UPlanSApo 30x 1,05 S
- Olympus UPLSAPO 40x 1.25 SIL
- Olympus UPLSAPO 60x 1.3 SIL
- Olympus UPlan SApochromat 100x 1.4 Oil
- Olympus UAPON 150x 1.45 TIRFM
- Illumination
- Transmitted Light (LED)
- Fluorescence (LED)
- Laser Diode 405 nm
- Laser Diode 445 nm
- Laser DPSS 488 nm
- Laser DPSS 514 nm
- Laser DPSS 561 nm
- Laser DPSS 638nm
- Laser DPSS 726nm
- Detection
- Spinning disc scan head Yokogawa CSU-W1 (4000rpm) with two pinhole discs (50um and 25um) and
- simultaneous dual camera detection: Camera 1 (Master): Andor iXon 888 Ultra with fringe suppression
- Camera 2 (Slave): Andor iXon 888 Ultra
- Reflectors
- CSU-W scan head:
- 3 main DM: Tripple (for 445nm
- 515nm
- 640nm laser)
- Quad (405nm
- 488nm
- 561nm
- 640nm); Penta (405nm
- 488nm
- 561nm
- 640nm
- 720nm)
FW1 (Master): Pos(1) LP568 - Pos(2) 525/50
- Pos(3) 617/73
- Pos(4) 607/36
- Pos(5) 685/40
- Pos(6) 785/62
- Pos(7) pol
- Pos(8) LP514
- Pos(9) 542/27
- Pos(10) 452/45
FW1 (Slave): Pos(1) 465/30 - Pos(2) 541/30
- Pos(3) 525/50
- Pos(4) 479/40
- Pos(5) pol
- Pos(6) - Pos(10) empty
- reflectors in IX83 stand:
DAPI set (Ex 377/50 - DM 409
- Em 447/60)
- GFP set (Ex 472/30
- DM 495
- Em 520/35)
- RFP set (Ex 562/40
- DM 593
- Em 624/40)
- CY5 set (Ex 628/40
- DM 660
- Em 692/40)
- Analyzer
- DM for use with Mosaic
- Software
- Andor iQ version 3.4.1
- MetaMorph version 7.8
- communication connections:
COM1(Port1): IX83 - COM3: CSU-WD
- COM4: Mosaic shutter
- COM6: prior XY stage
- COM7: 16 channel CooLED
Device: Mosaic- Device1: Mosaic laser
- DDA06/16 DAC board: Piezo
- trigger box
- ILE3082M-ILE3082S: ILE master
- slave
- Incubation
- Cage incubator (T+CO2)
Link to Further Details
https://www.biodip.de/wiki/SD4_-_Andor_Revolution_WD_Borealis_Mosaic
Points of Contact
Images
| Preview | Description |
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SD4 system.jpg |
Last Update
Last updated at: 8 April 2018 at 03:11:08