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Real-Time Confocal Microscope (SD4 - Andor Revolution WD Borealis Mosaic) Olympus IX83

Informationen

Name: Real-Time Confocal Microscope (SD4 - Andor Revolution WD Borealis Mosaic)
Hersteller: Olympus
Modell: IX83
Einrichtung: Abteilung für Lichtmikroskopie (LMF)
Partner: Max-Planck-Institut für Molekulare Zellbiologie und Genetik (MPI-CBG)

Beschreibung

Category
Light Microscope
Overview
Fast, "real-time" spinning disc confocal imaging with photo-activation capability. Fast imaging of large field of view and large tile scans with even illumination.
Suitable for confocal imaging, photo-activation using 405 laser with high temporal and spatial resolution; fast imaging of large samples over a long period of time
Features
Inverted stand
motorized with ZDC (hardware autofocus and focus search)
Prior NanoScanZ (400um travel range)
Prior ProScanIII xy scanning stage
Objectives
Olympus UPlan SApochromat 10x 0.4
Olympus UPlanSApo 30x 1,05 S
Olympus UPLSAPO 40x 1.25 SIL
Olympus UPLSAPO 60x 1.3 SIL
Olympus UPlan SApochromat 100x 1.4 Oil
Olympus UAPON 150x 1.45 TIRFM
Illumination
Transmitted Light (LED)
Fluorescence (LED)
Laser Diode 405 nm
Laser Diode 445 nm
Laser DPSS 488 nm
Laser DPSS 514 nm
Laser DPSS 561 nm
Laser DPSS 638nm
Laser DPSS 726nm
Detection
Spinning disc scan head Yokogawa CSU-W1 (4000rpm) with two pinhole discs (50um and 25um) and
simultaneous dual camera detection: Camera 1 (Master): Andor iXon 888 Ultra with fringe suppression
Camera 2 (Slave): Andor iXon 888 Ultra
Reflectors
CSU-W scan head:
3 main DM: Tripple (for 445nm
515nm
640nm laser)
Quad (405nm
488nm
561nm
640nm); Penta (405nm
488nm
561nm
640nm
720nm)
FW1 (Master): Pos(1) LP568
Pos(2) 525/50
Pos(3) 617/73
Pos(4) 607/36
Pos(5) 685/40
Pos(6) 785/62
Pos(7) pol
Pos(8) LP514
Pos(9) 542/27
Pos(10) 452/45
FW1 (Slave): Pos(1) 465/30
Pos(2) 541/30
Pos(3) 525/50
Pos(4) 479/40
Pos(5) pol
Pos(6) - Pos(10) empty
reflectors in IX83 stand:
DAPI set (Ex 377/50
DM 409
Em 447/60)
GFP set (Ex 472/30
DM 495
Em 520/35)
RFP set (Ex 562/40
DM 593
Em 624/40)
CY5 set (Ex 628/40
DM 660
Em 692/40)
Analyzer
DM for use with Mosaic
Software
Andor iQ version 3.4.1
MetaMorph version 7.8
communication connections:
COM1(Port1): IX83
COM3: CSU-WD
COM4: Mosaic shutter
COM6: prior XY stage
COM7: 16 channel CooLED

Device: Mosaic
Device1: Mosaic laser
DDA06/16 DAC board: Piezo
trigger box
ILE3082M-ILE3082S: ILE master
slave
Incubation
Cage incubator (T+CO2)

Link zu weiteren Informationen

https://www.biodip.de/wiki/SD4_-_Andor_Revolution_WD_Borealis_Mosaic

Ansprechpartner

LMF
E-Mail:
Telefon:
+49 351 210-2345
Fax:
+49 351 210-1078

Bilder

Letztes Update

Zuletzt aktualisiert am: 8. April 2018 um 03:11:08