View all instruments of this unit Wide-Field Microscope (W2 - DeltaVision Elite) Olympus IX71
Basic Information
| Name: | Wide-Field Microscope (W2 - DeltaVision Elite) | |
| Manufacturer: | Olympus | |
| Model: | IX71 | |
| Facility: | Light Microscopy (LMF) |
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| Partner: | Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG) |
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| Inventory number: | MOZGO ???? IP???? PC ID ??? | |
Description
- Category
- Light Microscope
- Overview
- High end widefield fluorescence / transmitted light (DIC) microscope with built in deconvolution. New generation motorised stage, Ultimate focus hardware autofocus system, and environmental control.
- Suitable for Live cell imaging, time lapse, multi point, multi-well plates. Tile scanning large samples. Scanning multi-well plates; High resolution deconvolution fluorescence microscopy.
- Features
- Inverted stand
- hybrid: partly motorized
- partly manual
- with Applied Precision add on equipment: new generation motorized stage
- ULtimate focus
- weather station black environmental control box
- with custom built Co2 control.
- Built in deconvolution software and optimised/selected precision optical hardware
- AOI scan (hardware z fast sum projection)
- tile scan
- multi point scan
- env control
- Easy to use software
- Much faster for large field of view than single point scanning confocal
- Blue Galss / UV filter in excitation path for live cell imaging
- ND filters for gentle illumination of living samples.
- Objectives
- Olympus UPlan SApochromat 10x 0.4
- Olympus UPlan SApochromat 20x 0.75
- Olympus UApochromat/340 40x 1.35 Oil
- Olympus Plan ApochromatN 60x 1.42 Oil
- Olympus UPlan SApochromat 100x 1.4 Oil
- Olympus UApochromat 150x 1.45 Oil TIRFM
- Olympus UPlan Apochromat 60x 1.20 Water
- Olympus UPlan SApochromat 100x 1.4 Oil
- Illumination
- SSI-lumencor
- Transmitted Light (LED)
- Detection
- PCO sCMOS
Roper Evolve EMCCD
- Reflectors
- [[reflectors::Separate excitation selection
- dichromatic mirrors and emission filter wheels (one for eyepiece and also camera) Fast 10 position fluo emitter wheel in front of camera
- Dichromatic mirrors for 1) DAPI/FITC/TRITC/Cy5
- 2) GFP/mCherry
- 3) CFP/YFP
- 4) DAPI/FITC/A594/Cy5
- see File:Filter Positions Hard Coat 10posEM-7posEP-DichroMs 2011.pdf]]
- Software
- Applied Precision SoftWorx running on a Cent OS Linux pc
- Hardware controlled indirectly via a windows XP machine (ICMIC) also on the system.
- Incubation
- Cage incubator (T+CO2)
Link to Further Details
Points of Contact
Images
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W2 - DeltaVision Core.jpg |
Last Update
Last updated at: 8 April 2018 at 03:11:14