Dresden
Technologieportal

 
Ihr Zugang zu Forschungsinfrastruktur und Know-how
de|en
Alle Geräte dieser Einrichtung anzeigen

Wide-Field Microscope (W2 - DeltaVision Elite) Olympus IX71

Informationen

Name: Wide-Field Microscope (W2 - DeltaVision Elite)
Hersteller: Olympus
Modell: IX71
Einrichtung: Abteilung für Lichtmikroskopie (LMF)
Partner: Max-Planck-Institut für Molekulare Zellbiologie und Genetik (MPI-CBG)
Inventarnummer: MOZGO ???? IP???? PC ID ???

Beschreibung

Category
Light Microscope
Overview
High end widefield fluorescence / transmitted light (DIC) microscope with built in deconvolution. New generation motorised stage, Ultimate focus hardware autofocus system, and environmental control.
Suitable for Live cell imaging, time lapse, multi point, multi-well plates. Tile scanning large samples. Scanning multi-well plates; High resolution deconvolution fluorescence microscopy.
Features
Inverted stand
hybrid: partly motorized
partly manual
with Applied Precision add on equipment: new generation motorized stage
ULtimate focus
weather station black environmental control box
with custom built Co2 control.
Built in deconvolution software and optimised/selected precision optical hardware
AOI scan (hardware z fast sum projection)
tile scan
multi point scan
env control
Easy to use software
Much faster for large field of view than single point scanning confocal
Blue Galss / UV filter in excitation path for live cell imaging
ND filters for gentle illumination of living samples.
Objectives
Olympus UPlan SApochromat 10x 0.4
Olympus UPlan SApochromat 20x 0.75
Olympus UApochromat/340 40x 1.35 Oil
Olympus Plan ApochromatN 60x 1.42 Oil
Olympus UPlan SApochromat 100x 1.4 Oil
Olympus UApochromat 150x 1.45 Oil TIRFM
Olympus UPlan Apochromat 60x 1.20 Water
Olympus UPlan SApochromat 100x 1.4 Oil
Illumination
SSI-lumencor
Transmitted Light (LED)
Detection
PCO sCMOS
Roper Evolve EMCCD
Reflectors
[[reflectors::Separate excitation selection
dichromatic mirrors and emission filter wheels (one for eyepiece and also camera) Fast 10 position fluo emitter wheel in front of camera
Dichromatic mirrors for 1) DAPI/FITC/TRITC/Cy5
2) GFP/mCherry
3) CFP/YFP
4) DAPI/FITC/A594/Cy5
see File:Filter Positions Hard Coat 10posEM-7posEP-DichroMs 2011.pdf]]
Software
Applied Precision SoftWorx running on a Cent OS Linux pc
Hardware controlled indirectly via a windows XP machine (ICMIC) also on the system.
Incubation
Cage incubator (T+CO2)

Link zu weiteren Informationen

https://www.biodip.de/wiki/W2_-_DeltaVision_Elite

Ansprechpartner

LMF
E-Mail:
Telefon:
+49 351 210-2345
Fax:
+49 351 210-1078

Bilder

Letztes Update

Zuletzt aktualisiert am: 8. April 2018 um 03:11:14