Multiphoton Laser Scanning Microscope (Zeiss LSM 780 NLO - DZNE M1) Zeiss Axio Examiner.Z1
Basic Information
Name: | Multiphoton Laser Scanning Microscope (Zeiss LSM 780 NLO - DZNE M1) | |
Manufacturer: | Zeiss | |
Model: | Axio Examiner.Z1 | |
Facility: | Light Microscopy Facility (LMF) | |
Partner: | German Center for Neurodegenerative Diseases Within The Helmholtz Association (DZNE) | |
Inventory number: | AIM System 2504000174 |
Description
- Category
- Light Microscope
- Overview
- Zeiss LSM 780 NLO
- Suitable for imaging of thick specimens and fast bleaching samples. Spectral imaging, routine dye combinations, tile scan, multi position scan
- Multiphoton Laser Scanning Microscope with a tunable pulsed infra-red laser (Chameleon Vision II) for multiphoton excitation.
- Single photon point scanning confocal system with two conventional PMTs and one spectral GaAsP detector and transmitted light detector. Motorized stage for multi-point acquisition"Zeiss LSM 780 NLO
- Multiphoton Laser Scanning Microscope with a tunable pulsed infra-red laser (Chameleon Vision II) for multiphoton excitation.
- Single photon point scanning confocal system with two conventional PMTs and one spectral GaAsP detector and transmitted light detector. Motorized stage for multi-point acquisition" cannot be used as a page name in this wiki.
- Features
- Upright stand
- motorized XY stage
- motorized Z-drive,
- simultaneous imaging
- sequential imaging
- line scan
- point scan
- Z stacks
- time-series
- advanced time-series
- averaging
- summarizing
- scan zoom and rotation
- tile scan
- multi position scan
- spectral scan
- Objectives
- Zeiss Plan-Apochromat 10x 0.45
- Zeiss Plan-Apochromat 20x 0.8
- Zeiss Plan-Apochromat 40x 0,95 korr.
- Zeiss LD LCI Plan-Apochromat 25x/0.8 Imm Korr DIC
- Zeiss LD W Plan-Apochromat 20x 1.0 Korr. VIS-IR
- Zeiss W Plan-Apochromat 20x/1.0
- Illumination
- Fluorescence (Metal Halide, HXP, 120W)
- Transmitted Light (Halogen)
- Laser Diode 405 nm
- Laser Argon Multiline 458, 488, 514 nm
- Laser DPSS 561 nm
- Laser HeNe 633 nm
- Laser Multiphoton Chameleon VisionII 690-1064nm
- Detection
- Single photon detection: 2 confocal PMTs with enhanced detection in the red range
- GaAsP spectral detector with 32 channels
- T-PMT
- dichromatic mirrors for excitation/emission splitting
- spectral prism sliders for emission detection range selection
- common pinhole changeable for each detector setting
- 2 photon detection: Two-channel non-descanned GaAsP detector (BiG) set up for emission spectra of EGFP (500-550nm) and RFP (575-610nm)
Two-channel non-descanned detector (NDD) set up for emission spectra of DAPI (447/60 nm) and Cy5 (700/75 nm)
- Reflectors
- Zeiss set 49: DAPI
- Zeiss set 38: GFP
Zeiss set 43: Cy3
- Software
- Zen "black" 2011
- Incubation
- Not available
Link to Further Details
Points of Contact
Images
Last Update
Last updated at: 8 April 2018 at 03:11:24