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Multiphoton Laser Scanning Microscope (Zeiss LSM 780 NLO - DZNE M1) Zeiss Axio Examiner.Z1

Basic Information

Name: Multiphoton Laser Scanning Microscope (Zeiss LSM 780 NLO - DZNE M1)
Manufacturer: Zeiss
Model: Axio Examiner.Z1
Facility: Light Microscopy Facility (LMF)
Partner: German Center for Neurodegenerative Diseases Within The Helmholtz Association (DZNE)
Inventory number: AIM System 2504000174

Description

Category
Light Microscope
Overview
Zeiss LSM 780 NLO
Suitable for imaging of thick specimens and fast bleaching samples. Spectral imaging, routine dye combinations, tile scan, multi position scan
Multiphoton Laser Scanning Microscope with a tunable pulsed infra-red laser (Chameleon Vision II) for multiphoton excitation.
Single photon point scanning confocal system with two conventional PMTs and one spectral GaAsP detector and transmitted light detector. Motorized stage for multi-point acquisition"Zeiss LSM 780 NLO
Multiphoton Laser Scanning Microscope with a tunable pulsed infra-red laser (Chameleon Vision II) for multiphoton excitation.
Single photon point scanning confocal system with two conventional PMTs and one spectral GaAsP detector and transmitted light detector. Motorized stage for multi-point acquisition" cannot be used as a page name in this wiki.
Features
Upright stand
motorized XY stage
motorized Z-drive,
simultaneous imaging
sequential imaging
line scan
point scan
Z stacks
time-series
advanced time-series
averaging
summarizing
scan zoom and rotation
tile scan
multi position scan
spectral scan
Objectives
Zeiss Plan-Apochromat 10x 0.45
Zeiss Plan-Apochromat 20x 0.8
Zeiss Plan-Apochromat 40x 0,95 korr.
Zeiss LD LCI Plan-Apochromat 25x/0.8 Imm Korr DIC
Zeiss LD W Plan-Apochromat 20x 1.0 Korr. VIS-IR
Zeiss W Plan-Apochromat 20x/1.0
Illumination
Fluorescence (Metal Halide, HXP, 120W)
Transmitted Light (Halogen)
Laser Diode 405 nm
Laser Argon Multiline 458, 488, 514 nm
Laser DPSS 561 nm
Laser HeNe 633 nm
Laser Multiphoton Chameleon VisionII 690-1064nm
Detection
Single photon detection: 2 confocal PMTs with enhanced detection in the red range
GaAsP spectral detector with 32 channels
T-PMT
dichromatic mirrors for excitation/emission splitting
spectral prism sliders for emission detection range selection
common pinhole changeable for each detector setting
2 photon detection: Two-channel non-descanned GaAsP detector (BiG) set up for emission spectra of EGFP (500-550nm) and RFP (575-610nm)
Two-channel non-descanned detector (NDD) set up for emission spectra of DAPI (447/60 nm) and Cy5 (700/75 nm)
Reflectors
Zeiss set 49: DAPI
Zeiss set 38: GFP
Zeiss set 43: Cy3
Software
Zen "black" 2011
Incubation
Not available

Link to Further Details

https://www.biodip.de/wiki/Zeiss_LSM_780_NLO_-_DZNE_M1

Points of Contact

Mrs. Silke White (Facility Leader)
Email:
Phone:
+49 (0)351 / 210463-716

Images

Last Update

Last updated at: 8 April 2018 at 03:11:24