Multiphoton Laser Scanning Microscope (Zeiss LSM 780 NLO - DZNE M1) Zeiss Axio Examiner.Z1

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Name:	Multiphoton Laser Scanning Microscope (Zeiss LSM 780 NLO - DZNE M1)
Hersteller:	Zeiss
Modell:	Axio Examiner.Z1
Einrichtung:	Lichtmikroskopie (LMF)
Partner:	Deutsches Zentrum für Neurodegenerative Erkrankungen in der Helmholtz-Gemeinschaft (DZNE)
Inventarnummer:	AIM System 2504000174

Overview: Zeiss LSM 780 NLO; Suitable for imaging of thick specimens and fast bleaching samples. Spectral imaging, routine dye combinations, tile scan, multi position scan; Multiphoton Laser Scanning Microscope with a tunable pulsed infra-red laser (Chameleon Vision II) for multiphoton excitation.; Single photon point scanning confocal system with two conventional PMTs and one spectral GaAsP detector and transmitted light detector. Motorized stage for multi-point acquisition"Zeiss LSM 780 NLO; Multiphoton Laser Scanning Microscope with a tunable pulsed infra-red laser (Chameleon Vision II) for multiphoton excitation.; Single photon point scanning confocal system with two conventional PMTs and one spectral GaAsP detector and transmitted light detector. Motorized stage for multi-point acquisition" cannot be used as a page name in this wiki.

Features: Upright stand; motorized XY stage; motorized Z-drive,; simultaneous imaging; sequential imaging; line scan; point scan; Z stacks; time-series; advanced time-series; averaging; summarizing; scan zoom and rotation; tile scan; multi position scan; spectral scan

Objectives: Zeiss Plan-Apochromat 10x 0.45; Zeiss Plan-Apochromat 20x 0.8; Zeiss Plan-Apochromat 40x 0,95 korr.; Zeiss LD LCI Plan-Apochromat 25x/0.8 Imm Korr DIC; Zeiss LD W Plan-Apochromat 20x 1.0 Korr. VIS-IR; Zeiss W Plan-Apochromat 20x/1.0

Illumination: Fluorescence (Metal Halide, HXP, 120W); Transmitted Light (Halogen); Laser Diode 405 nm; Laser Argon Multiline 458, 488, 514 nm; Laser DPSS 561 nm; Laser HeNe 633 nm; Laser Multiphoton Chameleon VisionII 690-1064nm

Detection: Single photon detection: 2 confocal PMTs with enhanced detection in the red range; GaAsP spectral detector with 32 channels; T-PMT; dichromatic mirrors for excitation/emission splitting; spectral prism sliders for emission detection range selection; common pinhole changeable for each detector setting; 2 photon detection: Two-channel non-descanned GaAsP detector (BiG) set up for emission spectra of EGFP (500-550nm) and RFP (575-610nm)
Two-channel non-descanned detector (NDD) set up for emission spectra of DAPI (447/60 nm) and Cy5 (700/75 nm)

Mrs. Silke White (Facility Leader)

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Telefon:

+49 (0)351 / 210463-716

Vorschau	Beschreibung
	02 LSM780NLO DZNE.jpg

Zuletzt aktualisiert am: 8. April 2018 um 03:11:24