The Isolera Spectra Four by Biotage is an advanced flash purification system allowing a fast and cost effective isolation of compounds from reaction mixtures.

The Isolera^TM Spektra Four by Biotage is an advanced flash purification system allowing a fast and cost effective isolation of compounds from reaction mixtures. A self-explanatory wizard determines a gradient based on TLC results. Subsequently, the gradient can be freely adapted to further optimize the separation process (e.g. step-gradients). Apart from corrosive solvents there are no restrictions on the used solvents and their quality. Up to four different solvents (quattro-binary gradients) may be used within a single run. An automatic baseline correction allows the detection of weekly absorbing substances even in a strongly absorbing mobile phase. Automated collection in into test tubes (20ml or 50ml) above a chosen absorbance threshold or in collect-all mode. Recognition of not baseline separated peaks results in a fraction change (also manually possible) to achieve maximum purification.       

Specifications:

Flowrate: 1-200ml/min                            

Detector: UV-Vis 200-800nm                        

UV Collection Modes: Single/dual/λ-All wavelengths (variable UV and UV-Vis)        

Pressure limit: 10bar                                    

Amount per run: milligrams to approx. 10g

Available cartridges: 10g, 25g, 50g, 100g and 300g silica SNAP KP-Sil (refillable), 100g SNAP Ultra C18 (reverse phase)