View all services of this unit RNA sequencing library preparation
Basic Information
| Name: | RNA sequencing library preparation | |
| Facility: | DRESDEN-concept Genome Center (DcGC) | |
| Partner: | Technische Universität Dresden (TUD) |
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| Partner: | Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG) |
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| Partner: | University Hospital Carl Gustav Carus Dresden (UKD) |
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Description
RNA-Sequencing
- Poly dT based mRNA isolation from total RNA
- Barcoded strand- and non-strandspecific RNA-Seq library preparation for single read and paired end reads sequencing (? 3 ?g total RNA)
- Low input RNA-Seq library preparation (< 3 ?g input) down to 500 ng established and further optimized in respect of maximizing the number of exonic reads, minimizing the number of intergenic reads
- With FACS sorting (see FACS Facility) combined RNA-Seq workflow enabling preparations of less than 500.000 cells (still in test phase, with 1 Mio cells it works perfect)
Link to Further Details
http://www.biotec.tu-dresden.de/technology-platform/deep-sequencing.html
Points of Contact
Dr. Andreas Dahl / Dr. Sylke Winkler
Web:
Email:
Phone:
+49 (0)351 458-82351 / +49 (0)351 210 2828
Last Update
Last updated at: 13 July 2017 at 11:54:16