Alle Dienstleistungen dieser Einrichtung anzeigen RNA sequencing library preparation
Informationen
| Name: | RNA sequencing library preparation | |
| Einrichtung: | DRESDEN-concept Genome Center (DcGC) | |
| Partner: | Technische Universität Dresden (TUD) |
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| Partner: | Max-Planck-Institut für Molekulare Zellbiologie und Genetik (MPI-CBG) |
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| Partner: | Universitätsklinikum Carl Gustav Carus Dresden (UKD) |
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Beschreibung
RNA-Sequencing
- Poly dT based mRNA isolation from total RNA
- Barcoded strand- and non-strandspecific RNA-Seq library preparation for single read and paired end reads sequencing (? 3 ?g total RNA)
- Low input RNA-Seq library preparation (< 3 ?g input) down to 500 ng established and further optimized in respect of maximizing the number of exonic reads, minimizing the number of intergenic reads
- With FACS sorting (see FACS Facility) combined RNA-Seq workflow enabling preparations of less than 500.000 cells (still in test phase, with 1 Mio cells it works perfect)
Link zu weiteren Informationen
http://www.biotec.tu-dresden.de/technology-platform/deep-sequencing.html
Ansprechpartner
Dr. Andreas Dahl / Dr. Sylke Winkler
Web:
E-Mail:
Telefon:
+49 (0)351 458-82351 / +49 (0)351 210 2828
Letztes Update
Zuletzt aktualisiert am: 13. Juli 2017 um 11:54:16