Dresden
Technology Portal

 
Your access to research infrastructure and know-how
de|en
View all services of this unit

Generation and characterization of induced pluripotent stem cells (hiPS)

Basic Information

Name: Generation and characterization of induced pluripotent stem cells (hiPS)
Facility: Stem Cell Engineering
Partner: Technische Universit├Ąt Dresden (TUD)

Short Description

The facility generates intergation/transgene free human induced pluripotent stem (hiPS) cells from diverse kinds of somatic cells.

Description

The facility generates intergation/transgene free human induced pluripotent stem (hiPS) cells from diverse kinds of somatic cells including, but not limited to, cells from cord and peripheral blood, various fibroblasts, brain endothelial cells.

 

The generated iPSCs are characterized for pluripotency using the following markers:

  

  • FLOW analysis of pluripotency markers
  • Quantitative real time PCR analysis for pluripotency markers
  • Quantitative real time PCR analysis for spontaneous and/or cytokine/growth factor assisted in vitro three germ layer differentiations
  • Short Tandem Repeat (STR) analysis
  • Karyotyping
  • Immunochemistry of pluripotency markers
  • Immunochemistry of three germ layer markers
  • Mycoplasma testing
  • Post Thaw viability

 

The customer of the facility chooses the number/level of characterization.

Points of Contact

Katrin Neumann
Email:
Phone:
+49 (0)351 458-82156

Images

Last Update

Last updated at: 5 November 2018 at 11:47:42