The facility generates intergation/transgene free human induced pluripotent stem (hiPS) cells from diverse kinds of somatic cells including, but not limited to, cells from cord and peripheral blood, various fibroblasts, brain endothelial cells.

The generated iPSCs are characterized for pluripotency using the following markers:

• FLOW analysis of pluripotency markers

• Quantitative real time PCR analysis for pluripotency markers

• Quantitative real time PCR analysis for spontaneous and/or cytokine/growth factor assisted in vitro three germ layer differentiations

• Short Tandem Repeat (STR) analysis

• Karyotyping

• Immunochemistry of pluripotency markers

• Immunochemistry of three germ layer markers

• Mycoplasma testing

• Post Thaw viability

The customer of the facility chooses the number/level of characterization.