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Total Internal Reflection Microscope (W1 - Olympus TIRF) Olympus IX71

Informationen

Name: Total Internal Reflection Microscope (W1 - Olympus TIRF)
Hersteller: Olympus
Modell: IX71
Einrichtung: Abteilung für Lichtmikroskopie (LMF)
Partner: Max-Planck-Institut für Molekulare Zellbiologie und Genetik (MPI-CBG)
Inventarnummer: 404504 10.1.145.13 AIM 2601000564

Beschreibung

Category
Light Microscope
Overview
Olympus TIRF setup.
Suitable for membrane trafficking studies; single molecule imaging; life yeast experiments; studies of cytoskeleton dynamics
Features
Inverted stand
manual
manual XY stage
custom-built TIRF condenser
manual TIRF angle adjustment
Objectives
Olympus UPlanSApochromat 10x 0.4
Olympus MPlanFluarN 100x 0.9
Olympus PlanApochromat 100x 1.45 Oil TIRFM
Olympus UApochromat 150x 1.45 Oil TIRFM
Olympus Apo N 100x 1.70 HIOil TIRFM
Illumination
Transmitted Light (Halogen)
Fluorescence (Metal Halide, X-Cite, 120W)
Laser DPSS 491 nm
Laser DPSS 561 nm
Detection
[[detection::Andor iXon Ultra 897 BV back illuminated EMCCD spec sheet;
pixel size of EMCCD chip: 16um;
image pixel size (measured June 29th
2012): with 10x objective: 1.592um/px (1x optovar)
0.795um/px (2x optovar)
with 100x objective: 0.159um/px (1x optovar)
0.080um/px (2x optovar); with 150x objective: 0.104um/px (1x optovar)
0.052um/px (2x optovar)]]
Reflectors
Hamamatsu W-View image splitter:
two matching dichromatic mirrors: 590LP + 590SP
five emitters: 520/15
525/30
617/73
628/40
Z491/561 M-TIRF
microscope filter turret:
position 1: for TIRF 488Ex/DC500/LP505Em
2: for TIRF Z488Ex/DCZDC488/BP 520/40
3: empty
4: tripple DC for 491
561 and 640 laser excitation
no emitter!
5: dual Ex and DC for 491 and 561 laser excitation
no emitter!
6: only DC and EM LP 575
Software
[[software::iQ 2.9 + iQ 3.2 iQ3 user guide
with Python IDE]]
Incubation
Stage incubator (T+CO2) + objective heater

Link zu weiteren Informationen

https://www.biodip.de/wiki/W1_-_Olympus_TIRF

Ansprechpartner

LMF
E-Mail:
Telefon:
+49 351 210-2345
Fax:
+49 351 210-1078

Bilder

Letztes Update

Zuletzt aktualisiert am: 8. April 2018 um 03:11:13