View all instruments of this unit Total Internal Reflection Microscope (W1 - Olympus TIRF) Olympus IX71
Basic Information
| Name: | Total Internal Reflection Microscope (W1 - Olympus TIRF) | |
| Manufacturer: | Olympus | |
| Model: | IX71 | |
| Facility: | Light Microscopy (LMF) |
|
| Partner: | Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG) |
|
| Inventory number: | 404504 10.1.145.13 AIM 2601000564 | |
Description
- Category
- Light Microscope
- Overview
- Olympus TIRF setup.
- Suitable for membrane trafficking studies; single molecule imaging; life yeast experiments; studies of cytoskeleton dynamics
- Features
- Inverted stand
- manual
- manual XY stage
- custom-built TIRF condenser
- manual TIRF angle adjustment
- Objectives
- Olympus UPlanSApochromat 10x 0.4
- Olympus MPlanFluarN 100x 0.9
- Olympus PlanApochromat 100x 1.45 Oil TIRFM
- Olympus UApochromat 150x 1.45 Oil TIRFM
- Olympus Apo N 100x 1.70 HIOil TIRFM
- Illumination
- Transmitted Light (Halogen)
- Fluorescence (Metal Halide, X-Cite, 120W)
- Laser DPSS 491 nm
- Laser DPSS 561 nm
- Detection
- [[detection::Andor iXon Ultra 897 BV back illuminated EMCCD spec sheet;
pixel size of EMCCD chip: 16um; - image pixel size (measured June 29th
- 2012): with 10x objective: 1.592um/px (1x optovar)
- 0.795um/px (2x optovar)
- with 100x objective: 0.159um/px (1x optovar)
- 0.080um/px (2x optovar); with 150x objective: 0.104um/px (1x optovar)
- 0.052um/px (2x optovar)]]
- Reflectors
- Hamamatsu W-View image splitter:
two matching dichromatic mirrors: 590LP + 590SP - five emitters: 520/15
- 525/30
- 617/73
- 628/40
- Z491/561 M-TIRF
microscope filter turret:
position 1: for TIRF 488Ex/DC500/LP505Em - 2: for TIRF Z488Ex/DCZDC488/BP 520/40
- 3: empty
- 4: tripple DC for 491
- 561 and 640 laser excitation
- no emitter!
- 5: dual Ex and DC for 491 and 561 laser excitation
- no emitter!
- 6: only DC and EM LP 575
- Software
- [[software::iQ 2.9 + iQ 3.2 iQ3 user guide
with Python IDE]]
- Incubation
- Stage incubator (T+CO2) + objective heater
Link to Further Details
Points of Contact
Images
| Preview | Description |
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MPI W1 TIRF.jpg |
Last Update
Last updated at: 8 April 2018 at 03:11:13