Pluripotent stem cells can be engineered using CRISPR/Cas9 technology or other molecular genetic techniques to generate various sublines with specific features:

• CRISPR/Cas9 assisted targeting of the human safe harbor locus AAVS1 to stably integrate (inducible) expression constructs
• CRISPR/Cas9 assisted targeting of endogenous genes to generate fluorescent reporters, protein fusions etc.
• Flp or Cre mediated excision of selection cassettes after targeting
• PiggyBAC transposition for stable integration of reporter or expression constructs
• gene knockouts by CRISPR/Cas9
• repair or mutation of single or few nucleotides (e.g. disease associated point mutations) to generate isogenic pairs of control and disease iPSC lines by:

• Homology directed repair (HDR) using Cas9-NLS protein, in vitro transcribed sgRNA and ssDNA oligonucleotides as template.
• Base editing using sgRNAs and Adenine or Cytosine base editor (ABE or CBE) mRNA 
• NEW: Prime editing using pegRNA and prime editor (PE) mRNA