Wide-Field Microscope (WF Screening System, BIOTEC)

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Name:	Wide-Field Microscope (WF Screening System, BIOTEC)
Einrichtung:	Lichtmikroskopie (LMF)
Partner:	Technische Universität Dresden (TUD)
Inventarnummer:	25007452

Overview: It is a high-content fast widefield screening microscope capable of automated imaging of fluorescence, transmitted light and phase contrast with a large field of view. It supplies a laser-based autofocus and provides an environmental control (T, CO2, humidity) unit. Thus it is suitable for fixed as well as for live cell imaging. The stage can be moved with 100 nm precision in x, y, and z direction. An universal plate holder provides flexibility from slides up to 1536 well plates."It is a high-content fast widefield screening microscope capable of automated imaging of fluorescence, transmitted light and phase contrast with a large field of view. It supplies a laser-based autofocus and provides an environmental control (T, CO2, humidity) unit. Thus it is suitable for fixed as well as for live cell imaging. The stage can be moved with 100 nm precision in x, y, and z direction. An universal plate holder provides flexibility from slides up to 1536 well plates." cannot be used as a page name in this wiki.; Suitable for high content screening of fluorescently labeled or unlabeled cells, either alive or fixed.

Objectives: Nikon PlanApo Lambda 2x 0.1 WD 8.5 mm; Nikon PlanApo Lambda 4x 0.2 WD 20 mm; Nikon PlanApo Lambda 10x 0.45 WD 4 mm; Nikon PlanApo Lambda 20x 0.75 WD 1 mm; Nikon PlanApo Lambda 40x 0.95; Nikon PlanFluor 10x 0.3 WD 16 mm; Nikon PlanFluor ELWD 20x 0.45 Ph1

Illumination: Transmitted Light (Nikon 100w Pillar Diascopic Illuminator); Fluorescence (Lumencor Spectra X light engine)

Reflectors: DAPI: EX 377/50 Dichroic 344-404 (R); 415-570 (T) EM 447/60; CFP: EX 438/24 Dichroic 426-450 (R); 467-600 (T) EM 483/32
Texas Red: EX 562/40 Dichroic 530-585 (R); 601-800 (T) EM 624/40
GFP: EX 472/30 Dichroic 442-488 (R); 502-730 (T) EM 520/35
Cy5: EX 628/40 Dichroic 594-651 (R); 669-726 (T) EM 692/40
Customized Cy5 will follow

Software: MetaXpress Imaging and Analysis software; AcuityXpress Analysis and Visualization software
MDCStore database and Microsoft SQL Express

Mrs. Dr. Hella Hartmann

Web:

E-Mail:

Telefon:

+49 (0)351 463-40230

Fax:

+49 (0) 351 463 40038

Vorschau	Beschreibung
	WF Screening System, BIOTEC.jpg

Zuletzt aktualisiert am: 8. April 2018 um 03:11:22