Category

Light Microscope

Overview

It is a high-content fast widefield screening microscope capable of automated imaging of fluorescence, transmitted light and phase contrast with a large field of view. It supplies a laser-based autofocus and provides an environmental control (T, CO2, humidity) unit. Thus it is suitable for fixed as well as for live cell imaging. The stage can be moved with 100 nm precision in x, y, and z direction. An universal plate holder provides flexibility from slides up to 1536 well plates.

cannot be used as a page name in this wiki.

Suitable for high content screening of fluorescently labeled or unlabeled cells, either alive or fixed.

Features

Inverted stand

-

hardware autofocus

Objectives

Nikon PlanApo Lambda 2x 0.1 WD 8.5 mm

Nikon PlanApo Lambda 4x 0.2 WD 20 mm

Nikon PlanApo Lambda 10x 0.45 WD 4 mm

Nikon PlanApo Lambda 20x 0.75 WD 1 mm

Nikon PlanApo Lambda 40x 0.95

Nikon PlanFluor 10x 0.3 WD 16 mm

Nikon PlanFluor ELWD 20x 0.45 Ph1

Illumination

Transmitted Light (Nikon 100w Pillar Diascopic Illuminator)

Fluorescence (Lumencor Spectra X light engine)

Detection

4.66 Megapixel scientific CMOS

Reflectors

DAPI: EX 377/50 Dichroic 344-404 (R)

415-570 (T) EM 447/60

CFP: EX 438/24 Dichroic 426-450 (R)

467-600 (T) EM 483/32
Texas Red: EX 562/40 Dichroic 530-585 (R)

601-800 (T) EM 624/40
GFP: EX 472/30 Dichroic 442-488 (R)

502-730 (T) EM 520/35
Cy5: EX 628/40 Dichroic 594-651 (R)

669-726 (T) EM 692/40
Customized Cy5 will follow

Software

MetaXpress Imaging and Analysis software

AcuityXpress Analysis and Visualization software
MDCStore database and Microsoft SQL Express

Incubation

Cage incubator (T+CO2)