View all instruments of this unit Wide-Field Microscope (WF Screening System, BIOTEC)
Basic Information
| Name: | Wide-Field Microscope (WF Screening System, BIOTEC) | |
| Facility: | Light Microscopy (LMF) |
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| Partner: | Technische Universität Dresden (TUD) |
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| Inventory number: | 25007452 | |
Description
- Category
- Light Microscope
- Overview
- It is a high-content fast widefield screening microscope capable of automated imaging of fluorescence, transmitted light and phase contrast with a large field of view. It supplies a laser-based autofocus and provides an environmental control (T, CO2, humidity) unit. Thus it is suitable for fixed as well as for live cell imaging. The stage can be moved with 100 nm precision in x, y, and z direction. An universal plate holder provides flexibility from slides up to 1536 well plates."It is a high-content fast widefield screening microscope capable of automated imaging of fluorescence, transmitted light and phase contrast with a large field of view. It supplies a laser-based autofocus and provides an environmental control (T, CO2, humidity) unit. Thus it is suitable for fixed as well as for live cell imaging. The stage can be moved with 100 nm precision in x, y, and z direction. An universal plate holder provides flexibility from slides up to 1536 well plates." cannot be used as a page name in this wiki.
- Suitable for high content screening of fluorescently labeled or unlabeled cells, either alive or fixed.
- Features
- Inverted stand
- -
- hardware autofocus
- Objectives
- Nikon PlanApo Lambda 2x 0.1 WD 8.5 mm
- Nikon PlanApo Lambda 4x 0.2 WD 20 mm
- Nikon PlanApo Lambda 10x 0.45 WD 4 mm
- Nikon PlanApo Lambda 20x 0.75 WD 1 mm
- Nikon PlanApo Lambda 40x 0.95
- Nikon PlanFluor 10x 0.3 WD 16 mm
- Nikon PlanFluor ELWD 20x 0.45 Ph1
- Illumination
- Transmitted Light (Nikon 100w Pillar Diascopic Illuminator)
- Fluorescence (Lumencor Spectra X light engine)
- Detection
- 4.66 Megapixel scientific CMOS
- Reflectors
- DAPI: EX 377/50 Dichroic 344-404 (R)
- 415-570 (T) EM 447/60
- CFP: EX 438/24 Dichroic 426-450 (R)
- 467-600 (T) EM 483/32
Texas Red: EX 562/40 Dichroic 530-585 (R) - 601-800 (T) EM 624/40
GFP: EX 472/30 Dichroic 442-488 (R) - 502-730 (T) EM 520/35
Cy5: EX 628/40 Dichroic 594-651 (R) - 669-726 (T) EM 692/40
Customized Cy5 will follow
- Software
- MetaXpress Imaging and Analysis software
- AcuityXpress Analysis and Visualization software
MDCStore database and Microsoft SQL Express
- Incubation
- Cage incubator (T+CO2)
Link to Further Details
Points of Contact
Images
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WF Screening System, BIOTEC.jpg |
Last Update
Last updated at: 8 April 2018 at 03:11:22