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Wide-Field Microscope (WF Screening System, BIOTEC)

Basic Information

Name: Wide-Field Microscope (WF Screening System, BIOTEC)
Facility: Light Microscopy (LMF)
Partner: Technische Universität Dresden (TUD)
Inventory number: 25007452

Description

Category
Light Microscope
Overview
It is a high-content fast widefield screening microscope capable of automated imaging of fluorescence, transmitted light and phase contrast with a large field of view. It supplies a laser-based autofocus and provides an environmental control (T, CO2, humidity) unit. Thus it is suitable for fixed as well as for live cell imaging. The stage can be moved with 100 nm precision in x, y, and z direction. An universal plate holder provides flexibility from slides up to 1536 well plates."It is a high-content fast widefield screening microscope capable of automated imaging of fluorescence, transmitted light and phase contrast with a large field of view. It supplies a laser-based autofocus and provides an environmental control (T, CO2, humidity) unit. Thus it is suitable for fixed as well as for live cell imaging. The stage can be moved with 100 nm precision in x, y, and z direction. An universal plate holder provides flexibility from slides up to 1536 well plates." cannot be used as a page name in this wiki.
Suitable for high content screening of fluorescently labeled or unlabeled cells, either alive or fixed.
Features
Inverted stand
-
hardware autofocus
Objectives
Nikon PlanApo Lambda 2x 0.1 WD 8.5 mm
Nikon PlanApo Lambda 4x 0.2 WD 20 mm
Nikon PlanApo Lambda 10x 0.45 WD 4 mm
Nikon PlanApo Lambda 20x 0.75 WD 1 mm
Nikon PlanApo Lambda 40x 0.95
Nikon PlanFluor 10x 0.3 WD 16 mm
Nikon PlanFluor ELWD 20x 0.45 Ph1
Illumination
Transmitted Light (Nikon 100w Pillar Diascopic Illuminator)
Fluorescence (Lumencor Spectra X light engine)
Detection
4.66 Megapixel scientific CMOS
Reflectors
DAPI: EX 377/50 Dichroic 344-404 (R)
415-570 (T) EM 447/60
CFP: EX 438/24 Dichroic 426-450 (R)
467-600 (T) EM 483/32
Texas Red: EX 562/40 Dichroic 530-585 (R)
601-800 (T) EM 624/40
GFP: EX 472/30 Dichroic 442-488 (R)
502-730 (T) EM 520/35
Cy5: EX 628/40 Dichroic 594-651 (R)
669-726 (T) EM 692/40
Customized Cy5 will follow
Software
MetaXpress Imaging and Analysis software
AcuityXpress Analysis and Visualization software
MDCStore database and Microsoft SQL Express
Incubation
Cage incubator (T+CO2)

Link to Further Details

https://www.biodip.de/wiki/WF_Screening_System,_BIOTEC

Points of Contact

Mrs. Dr. Hella Hartmann
Email:
Phone:
+49 (0)351 463-40230
Fax:
+49 (0) 351 463 40038

Images

Last Update

Last updated at: 8 April 2018 at 03:11:22