Characterization of iPSCs:

• ICC for Pluripotency Markers

  Immunocytochemistry (ICC) for pluripotency markers typically expressed in iPSCs: OCT4, SOX2, NANOG and TRA-1-60.

• Flow Cytometry for Pluripotency Markers

  Flow Cytometry for markers of the undifferetiated state typically expressed in iPSCs: OCT4, SOX2, SSEA-4 and TRA-1-60.

• 96-Gene qRT-PCR Array

  Quantitative RT-PCR Array for 96 genes involved in pluripotency, stem cell maintenance, differentiation and house keeping. 

• Trilineage Differentiation

  Differentiation of iPSC to derivatives of the 3 germ layers: ectoderm, endoderm and mesoderm. 

• Karyotyping

  Karyotype analysis by Giemsa staining of metaphase chromosomes.

• Mycoplasma Test

  PCR test for the most common contaminant in cell cultures - Mycoplasma spp.

• STR Analysis

  Short Tandem Repeat (STR) analysis by multiplex PCR for cell line identification.