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Real-Time Confocal Microscope (Grid LSM & WF, upr., BIOTEC) Zeiss Axio Imager

Basic Information

Name: Real-Time Confocal Microscope (Grid LSM & WF, upr., BIOTEC)
Manufacturer: Zeiss
Model: Axio Imager
Facility: Light Microscopy (LMF)
Partner: Technische Universität Dresden (TUD)
Inventory number: 143913

Description

Category
Light Microscope
Overview
The Vt-Infinity III is dedicated for high speed confocal imaging in XYZ. The laser lines allow GFP and RFP imaging, the HBO allows common dye combinations. An EMCCD allows very fast stream acquisition of low signal. A dual-view system allows simultaneous detection of GFP and RFP in confocal mode. The use of dipping lenses makes this system especially suitable for study of embryo development."The Vt-Infinity III is dedicated for high speed confocal imaging in XYZ. The laser lines allow GFP and RFP imaging, the HBO allows common dye combinations. An EMCCD allows very fast stream acquisition of low signal. A dual-view system allows simultaneous detection of GFP and RFP in confocal mode. The use of dipping lenses makes this system especially suitable for study of embryo development." cannot be used as a page name in this wiki.
Features
Upright stand
motorized XY stage
Z-Piezo stage
fluorescence
transmitted light with manual DIC
widefield fluoerescence imaging
confocal imaging
simultaneous GFP/RFP imaging
sequential imaging
Z stacks
time-series
advanced time-series
averaging
Objectives
Zeiss EC Plan-Neofluar 10x 0.3
Zeiss Achroplan 40x 0.8 W
Zeiss LD C-Apochromat 40x 1.1 W
Zeiss Plan-Apochromat 63x 1.4 Oil
Zeiss Plan-Apochromat 100x 1.4 Oil
Illumination
Fluorescence (Metal Halide, HXP, 120W)
Transmitted Light (Halogen)
Laser DPSS 488 nm
Laser DPSS 561 nm
Detection
dual ccd configuration:
Coolsnap ES for widefield fluorescence AND
Cascade II EMCCD for confocal sectioning (multi point illumination
variable pinhole size)
dual channel emission beamsplitter
Reflectors
Analysator
GFP : EX 470/40 
BS 495 
EM 525/50
FITC : EX 480/20 
BS 495 
EM 535/40
TxRed : EX 570/20 
BS 590 
EM 640/40
DAPI (A) : EX 350/50 
BS 400 
EM LP 460/50
Software
MetaMorph
Incubation
Stage incubator (T)

Link to Further Details

https://www.biodip.de/wiki/Grid_LSM_%26_WF,_upr.,_BIOTEC

Points of Contact

Mrs. Dr. Hella Hartmann
Email:
Phone:
+49 (0)351 463-40230
Fax:
+49 (0) 351 463 40038

Images

Last Update

Last updated at: 8 April 2018 at 03:10:48